Difference between revisions of "Team:Munich/Testing"

Line 17: Line 17:
 
}
 
}
  
/*Time for the CSS*/
 
* {margin: 0; padding: 0;}
 
body {background: #ccc;}
 
 
.slider{
 
width: 640px; /*Same as width of the large image*/
 
position: relative;
 
/*Instead of height we will use padding*/
 
padding-top: 320px; /*That helps bring the labels down*/
 
 
margin: 100px auto;
 
 
/*Lets add a shadow*/
 
}
 
 
 
/*Last thing remaining is to add transitions*/
 
.slider>img{
 
position: absolute;
 
left: 0; top: 0;
 
transition: all 0.5s;
 
}
 
 
.slider input[name='slide_switch'] {
 
display: none;
 
}
 
 
.slider label {
 
/*Lets add some spacing for the thumbnails*/
 
margin: 18px 0 0 18px;
 
 
float: left;
 
cursor: pointer;
 
transition: all 0.5s;
 
 
/*Default style = low opacity*/
 
}
 
 
.slider label img{
 
display: block;
 
}
 
 
/*Time to add the click effects*/
 
.slider input[name='slide_switch']:checked+label {
 
border-color: #666;
 
opacity: 1;
 
}
 
/*Clicking any thumbnail now should change its opacity(style)*/
 
/*Time to work on the main images*/
 
.slider input[name='slide_switch'] ~ img {
 
opacity: 0;
 
transform: scale(1.1);
 
}
 
/*That hides all main images at a 110% size
 
On click the images will be displayed at normal size to complete the effect
 
*/
 
.slider input[name='slide_switch']:checked+label+img {
 
opacity: 1;
 
transform: scale(1);
 
}
 
/*Clicking on any thumbnail now should activate the image related to it*/
 
 
/*We are done :)*/
 
 
</style>
 
</style>
 
</head></html>
 
</head></html>

Revision as of 22:12, 14 December 2017


Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics. Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

Awards & Achievements

First Runner-up

Best Diagnostics Project

Best Model

Best Applied Design

Best Hardware

Best Software

Nominated for Best Wiki

Nominated for Best Presentation

Nominated for Best Poster

Nominated for Best Integrated Human Practices

Nominated for Best Best Education & Public Engagement

Nominated for Best Measurement

Nominated for Best Composite Part

Nominated for Best Part Collection

Gallery