Difference between revisions of "Team:NCKU Tainan/Measurement"

(Prototype team page)
 
 
(13 intermediate revisions by 5 users not shown)
Line 1: Line 1:
−
{{NCKU_Tainan}}
+
{{NCKU_Tainan/Measurement_css}}{{NCKU_Tainan/Header}}{{NCKU_Tainan/animate_css}}
 
<html>
 
<html>
 +
<head>
  
−
<div class="column full_size judges-will-not-evaluate">
+
<script src="https://2017.igem.org/Template:NCKU_Tainan/js/main_js&action=raw&ctype=text/javascript"></script>  
−
<h3>★  ALERT! </h3>
+
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
−
</div>
+
−
<div class="clear"></div>
+
  
  
 +
<style>
 +
.vertical-container{
 +
  background-image: url('https://static.igem.org/mediawiki/2017/9/9d/T--NCKU_Tainan--hardware-background.png');
 +
  background-attachment: fixed;
 +
}
  
 +
</style>
  
 +
</head>
 +
<body>
  
  
−
<div class="column full_size">
+
<div class="container-fluid">
−
<h1>Measurement</h1>
+
  <div class="row">
 +
    <div class="col">
 +
      <div id="top">
 +
      </div>
 +
      <div id="category" class="vertical-container">
 +
          <h1 class="wet">Measurement</h1>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
<div id="paragraph">
 +
<div class="container-fluid">
 +
  <div class="row">
 +
    <div id="paragraph" class="paragraph col-md-8 col-md-offset-1 col-xs-offset-1 col-xs-10">
 +
      <h2 id="biobrick">BioBrick</h2>
 +
      <hr>
 +
      <p>
 +
        BBa_K381001 was first used by team BCCS-Bristol in iGEM 2010. This part encodes nitrate and nitrite sensitive promoter P<sub>yeaR</sub> with a GFP coding device and B0030 to create a nitrate-sensitive system which signals through expression of GFP. In 2010, the team BCCS-Bristol used it to sense the nitrate concentration in soil. Yet, our team think this part can do more than that. This year, our team decided to create a whole new measurement system for sensing the nitrate level in aquaculture water by using this part.
 +
      </p>
 +
      <h2 id="powder">How dose it work ? Lyophilized <i>E. coli</i> powder ?</h2>
 +
      <hr>
 +
      <p>
 +
        The nitrate in water will activate P<sub>yeaR</sub> promoter and lead to expression of GFP. We realize that liquid broth <i>E. coli</i> doesn’t work well in this system, having to wait for a long-time period to get the value, which is not accepted by fish farmers because a sudden increase of nitrate level will lead to the dead of the whole pool in an instant. Therefore, instead of liquid broth, we choose lyophilized <i>E. coli</i> powder as our platform. When water pumped into the cuvette, activity of lyophilized <i>E. coli</i> recovered and showed corresponding fluorescence intensity along with different nitrate level in merely 20 minutes. Comparing to 4 hours detection time in broth process, the time cost of lyophilized <i>E. coli</i> powder decreases dramatically.
 +
      </p>
 +
      <div class="row imagerow">
 +
        <div class="col-md-12">
 +
          <img src="https://static.igem.org/mediawiki/2017/6/65/NCKU-measurement-1.jpeg">
 +
          <p>
 +
            Figure 1 . The lyophilized <i>E. coli</i> powder
 +
          </p>
 +
        </div>
 +
      </div>
 +
      <h2 id="nitrate_sensor">Nitrate Sensor </h2>
 +
      <hr>
 +
      <p>
 +
        The BioBrick alone is not enough to create a fully functional measurement system, a sensing device is also one of the key element to perfect the system. After doing some research, we self-assembled a portable GFP sensing device.
 +
      </p>
 +
      <div class="row imagerow">
 +
        <div class="col-md-12">
 +
          <img src="https://static.igem.org/mediawiki/2017/d/d2/NCKU-measurement-2.png">
 +
          <p>
 +
            Figure 2 . Our idea of sensing the GFP expressed by the lyophilized <i>E. coli</i> powder
 +
          </p>
 +
        </div>
 +
      </div>
 +
      <h2 id="mechanism">The Mechanism </h2>
 +
      <hr>
 +
      <p>
 +
        The value of fluorescence is detected by our sensing device and recorded as voltage form. By cross-matching our data base and modelling results with voltage signal detected by device, we can evaluate the nitrate level in water accurately to 1 ppm interval. In a relatively short time period, 20 minutes , our users are able to know the nitrate level of the pond via our app.
 +
      </p>
 +
      <div class="row imagerow">
 +
        <div class="col-md-12">
 +
          <img src="https://static.igem.org/mediawiki/2017/9/91/NCKU-measurement-3.jpeg">
 +
          <p>
 +
            Figure 3 . The look of our sensing system when sensing the GFP expressed by the lyophilized <i>E. coli</i> powder.
 +
          </p>
 +
        </div>
 +
      </div>
 +
      <h2 id="advantages">Advantages</h2>
 +
      <hr>
 +
      <p>
 +
        Compared with the spectrophotometer in the lab, our sensing device is smaller, more portable and less energy consumed. Incorporating our data base with modelling result, we can assure the sensitivity and accuracy of our nitrate sensor.
 +
Last but not least, due to the portability of the sensing device and storability of the lyophilized <i>E. coli</i> powder, this method has a lot of potential to be commercialized.
  
−
<p>There are a lot of exciting parts in the Registry, but many parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
+
      </p>
 +
    </div>
 +
    <div id="sidemenu" class="col-md-2">
 +
      <div class="list-group">
 +
        <a onclick="scrollto('#biobrick')" class="list-group-item">BioBrick</a>
 +
        <hr>
 +
        <a onclick="scrollto('#powder')" class="list-group-item">How dose it work ?</a>
 +
        <hr>
 +
        <a onclick="scrollto('#nitrate_sensor')" class="list-group-item">Nitrate Sensor</a>
 +
        <hr>
 +
        <a onclick="scrollto('#mechanism')" class="list-group-item">The Mechanism</a>
 +
        <hr>
 +
        <a onclick="scrollto('#advantages')" class="list-group-item">Advantages</a>
 +
        <hr>
 +
        <a class="list-group-item top"><i  onclick="scrollto('#top')" class="fa fa-arrow-up fa-1x" aria-hidden="true"></i></a>
 +
      </div>
 +
    </div>
 +
  </div>
 
</div>
 
</div>
−
<div class="clear"></div>
 
  
−
<div class="column half_size">
 
−
<h3>Best Innovation in Measurement Special Prize</h3>
 
−
<p>If you've done excellent work in measurement, you should consider nominating your team for this special prize. Designing great measurement approaches for characterizing new parts or developing and implementing an efficient new method for characterizing thousands of parts are good examples.
 
−
<br><br>
 
−
To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Innovation in Measurement prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
 
−
<br><br>
 
−
You must also delete the message box on the top of this page to be eligible for this prize.
 
−
 
−
</p>
 
−
<br>
 
 
</div>
 
</div>
−
<div class="column half_size">
 
  
−
<h5>Inspiration</h5>
+
</body>
−
<p>You can look at what other teams did to get some inspiration! <br />
+
 
−
Here are a few examples:</p>
+
−
<ul>
+
−
<li><a href="https://2016.igem.org/Team:Stanford-Brown">2016 Stanford-Brown</a></li>
+
−
<li><a href="https://2016.igem.org/Team:Genspace">2016 Genspace</a></li>
+
−
<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
+
−
<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
+
−
</ul>
+
  
−
</div>
 
−
<div class="clear"></div>
 
 
</html>
 
</html>

Latest revision as of 11:32, 1 November 2017

Measurement

BioBrick

BBa_K381001 was first used by team BCCS-Bristol in iGEM 2010. This part encodes nitrate and nitrite sensitive promoter PyeaR with a GFP coding device and B0030 to create a nitrate-sensitive system which signals through expression of GFP. In 2010, the team BCCS-Bristol used it to sense the nitrate concentration in soil. Yet, our team think this part can do more than that. This year, our team decided to create a whole new measurement system for sensing the nitrate level in aquaculture water by using this part.

How dose it work ? Lyophilized E. coli powder ?

The nitrate in water will activate PyeaR promoter and lead to expression of GFP. We realize that liquid broth E. coli doesn’t work well in this system, having to wait for a long-time period to get the value, which is not accepted by fish farmers because a sudden increase of nitrate level will lead to the dead of the whole pool in an instant. Therefore, instead of liquid broth, we choose lyophilized E. coli powder as our platform. When water pumped into the cuvette, activity of lyophilized E. coli recovered and showed corresponding fluorescence intensity along with different nitrate level in merely 20 minutes. Comparing to 4 hours detection time in broth process, the time cost of lyophilized E. coli powder decreases dramatically.

Figure 1 . The lyophilized E. coli powder

Nitrate Sensor

The BioBrick alone is not enough to create a fully functional measurement system, a sensing device is also one of the key element to perfect the system. After doing some research, we self-assembled a portable GFP sensing device.

Figure 2 . Our idea of sensing the GFP expressed by the lyophilized E. coli powder

The Mechanism

The value of fluorescence is detected by our sensing device and recorded as voltage form. By cross-matching our data base and modelling results with voltage signal detected by device, we can evaluate the nitrate level in water accurately to 1 ppm interval. In a relatively short time period, 20 minutes , our users are able to know the nitrate level of the pond via our app.

Figure 3 . The look of our sensing system when sensing the GFP expressed by the lyophilized E. coli powder.

Advantages

Compared with the spectrophotometer in the lab, our sensing device is smaller, more portable and less energy consumed. Incorporating our data base with modelling result, we can assure the sensitivity and accuracy of our nitrate sensor. Last but not least, due to the portability of the sensing device and storability of the lyophilized E. coli powder, this method has a lot of potential to be commercialized.