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− | + | <img src="https://static.igem.org/mediawiki/2017/2/28/Npu-banner2.jpg"> | |
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</header> | </header> | ||
+ | |||
<div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;"> | <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;"> | ||
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<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
<a href="portfolio-item.html"> | <a href="portfolio-item.html"> | ||
− | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/ | + | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/ceaS2.png"> |
</a> | </a> | ||
<h3> | <h3> | ||
<a href="portfolio-item.html">Core Part</a> | <a href="portfolio-item.html">Core Part</a> | ||
</h3> | </h3> | ||
− | <h4>We use | + | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type |
− | We designed | + | 's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic |
− | </h4> | + | effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) |
+ | platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography) | ||
+ | and HTS(High throughput screening). | ||
+ | </h4> | ||
</div> | </div> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
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<a href="portfolio-item.html">System</a> | <a href="portfolio-item.html">System</a> | ||
</h3> | </h3> | ||
− | <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually. | + | <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient |
− | </h4> | + | to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, |
+ | we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually. | ||
+ | </h4> | ||
</div> | </div> | ||
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<a href="portfolio-item.html">Pathway</a> | <a href="portfolio-item.html">Pathway</a> | ||
</h3> | </h3> | ||
− | <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes for them.We introduced the | + | <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different |
+ | optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol | ||
+ | metabolism of the two bacteria, so that it could produce the target product acrylic acid using the | ||
+ | intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed | ||
+ | and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | ||
+ | and reduce the loss of bypass carbon flux.</h4> | ||
</div> | </div> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
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<a href="portfolio-item.html">Production</a> | <a href="portfolio-item.html">Production</a> | ||
</h3> | </h3> | ||
− | <h4>All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.<br> | + | <h4>All of the previous processes are to build the engineering strains which have a high production of acrylic |
− | Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.</h4> | + | acid that we need. In the subsequent fermentation, we also need to determine the best parameters |
+ | of the engineering strain.<br> Therefore, we selected the carbon source, Buffer, temperature, pH | ||
+ | and other conditions to optimize the cell production process.</h4> | ||
</div> | </div> | ||
</div> | </div> | ||
− | + | </div> | |
− | + | <!-- /.container --> | |
− | + | <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png"> | |
</div> | </div> | ||
− | <!-- /. | + | <!-- /.batu --> |
Revision as of 13:34, 21 October 2017