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polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average | polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average | ||
annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion | annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion | ||
− | US dollars | + | US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which |
− | is obtained by petroleum cracking) after multi-step treatment | + | is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, |
− | high energy consumption and | + | high energy consumption and a lack of sustainablility.<br> This year, we aim to use a greener and more environmentally- |
− | friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional | + | friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional |
− | chemical synthesis methods, Synbio is | + | chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than ethylene. |
</h4> | </h4> | ||
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<div class="row" style="padding-top:70px"> | <div class="row" style="padding-top:70px"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
− | <h2 class="page-header" align="center">We | + | <h2 class="page-header" align="center">We constructed our cell factory based on 4 levels, which are—</h2> |
<br> | <br> | ||
</div> | </div> | ||
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<a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a> | <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a> | ||
</h3> | </h3> | ||
− | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a | + | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type |
− | + | is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic | |
effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) | effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) | ||
− | platform and screened for | + | platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) |
and HTS(High throughput screening). | and HTS(High throughput screening). | ||
</h4> | </h4> | ||
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<h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient | <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient | ||
to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, | to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, | ||
− | we have | + | we have E. coli MG1655 and S. cerevisiae BY4741 tested individually. |
</h4> | </h4> | ||
</div> | </div> | ||
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</h3> | </h3> | ||
<h4>We need to design two different metabolic pathways for two different chassis organisms and propose different | <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different | ||
− | optimization schemes | + | optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol |
− | metabolism of the two | + | metabolism of the two organisms, so that they could produce the target product acrylic acid using the |
− | intermediates G3P and DHAP.Besides | + | intermediates G3P and DHAP. Besides the construction of the pathways, we also reconstructed |
and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | ||
− | and | + | and reduced the loss of by-pass carbon flux.</h4> |
</div> | </div> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
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<a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a> | <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a> | ||
</h3> | </h3> | ||
− | <h4>All of the previous processes | + | <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic |
− | acid | + | acid. In the subsequent fermentation, we also determined the best conditions |
− | of the | + | of the engineered microorganism strains.<br> Therefore, we selected to control the carbon source, buffer, temperature, pH |
and other conditions to optimize the cell production process.</h4> | and other conditions to optimize the cell production process.</h4> | ||
</div> | </div> |
Revision as of 18:41, 31 October 2017