Difference between revisions of "Team:NPU-China"

 
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                         polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 
                         polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 
                         annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
 
                         annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
−
                         US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which
+
                         US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which
−
                         is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution,
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                         is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution,
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                         high energy consumption and it is unsustainable.<br> This year, we aim to use a green and environmentally
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                         high energy consumption and a lack of sustainablility.<br><br> This year, we aim to use a greener and more environmentally-
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                         friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional
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                         friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional
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                         chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.
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                         chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene.
 
                     </h4>
 
                     </h4>
 
                    
 
                    
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             <div class="row" style="padding-top:70px">
 
             <div class="row" style="padding-top:70px">
 
                 <div class="col-md-12">
 
                 <div class="col-md-12">
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                     <h2 class="page-header" align="center">We construct cell factory based on 4 levels, which are—</h2>
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                     <h2 class="page-header" align="center">We constructed our cell factory based on 4 levels, which are—</h2>
 
                     <br>
 
                     <br>
 
                 </div>
 
                 </div>
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                         <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a>
 
                         <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a>
 
                     </h3>
 
                     </h3>
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                     <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type
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                     <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type
−
                         's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic
+
                         is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic
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                         effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design)
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                         effect of ceaS2 enzyme.<br><br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design)
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                         platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography)
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                         platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography)
 
                         and HTS(High throughput screening).
 
                         and HTS(High throughput screening).
 
                     </h4>
 
                     </h4>
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                     <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient
 
                     <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient
 
                         to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms,
 
                         to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms,
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                         we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually.
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                         we have E. coli MG1655 and S. cerevisiae BY4741 tested individually.
 
                     </h4>
 
                     </h4>
 
                 </div>
 
                 </div>
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                     </h3>
 
                     </h3>
 
                     <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different
 
                     <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different
−
                         optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol
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                         optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol
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                         metabolism of the two bacteria, so that it could produce the target product acrylic acid using the
+
                         metabolism of the two organisms, so that they could produce the target product acrylic acid using the
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                         intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed
+
                         intermediates G3P and DHAP.<br><br> Besides the construction of the pathways, we also reconstructed
 
                         and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway
 
                         and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway
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                         and reduce the loss of bypass carbon flux.</h4>
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                         and reduced the loss of by-pass carbon flux.</h4>
 
                 </div>
 
                 </div>
 
                 <div class="col-md-6 img-portfolio">
 
                 <div class="col-md-6 img-portfolio">
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                         <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a>
 
                         <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a>
 
                     </h3>
 
                     </h3>
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                     <h4>All of the previous processes are to build the engineering strains which have a high production of acrylic
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                     <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic
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                         acid that we need. In the subsequent fermentation, we also need to determine the best parameters
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                         acid. In the subsequent fermentation, we also determined the best conditions
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                         of the engineering strain.<br> Therefore, we selected the carbon source, Buffer, temperature, pH
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                         of the engineered microorganism strains.<br><br> Therefore, we selected to control the carbon source, buffer, temperature, pH
 
                         and other conditions to optimize the cell production process.</h4>
 
                         and other conditions to optimize the cell production process.</h4>
 
                 </div>
 
                 </div>

Latest revision as of 12:52, 1 November 2017

Abstract

Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, high energy consumption and a lack of sustainablility.

This year, we aim to use a greener and more environmentally- friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene.

We constructed our cell factory based on 4 levels, which are—


Core Part

We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic effect of ceaS2 enzyme.

We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).

System

Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, we have E. coli MG1655 and S. cerevisiae BY4741 tested individually.

Pathway

We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol metabolism of the two organisms, so that they could produce the target product acrylic acid using the intermediates G3P and DHAP.

Besides the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduced the loss of by-pass carbon flux.

Production

All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic acid. In the subsequent fermentation, we also determined the best conditions of the engineered microorganism strains.

Therefore, we selected to control the carbon source, buffer, temperature, pH and other conditions to optimize the cell production process.