Difference between revisions of "Team:NPU-China/Attributions"

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    <link href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet">
  
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<h3>★  ALERT! </h3>
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    <script src="https://cdn.bootcss.com/jquery/3.2.1/jquery.min.js"></script>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="column full_size">
 
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<h1>Attributions</h1>
 
  
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.
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<body>
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<br><br>
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This is a bronze medal requirement. Please see the <a href="https://2017.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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                </a>
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                    <li class="active"> <a href="https://2017.igem.org/Team:NPU-China">Home</a> </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li>
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                        </ul>
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                    </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li>
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                        </ul>
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                    </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> </li>
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                        </ul>
 +
                    </li>
 +
                    <li> <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> </li>
 +
                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a>
 +
                        <ul class="dropdown-menu">
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li>
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                        </ul>
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                    </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li>
  
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li>
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                        </ul>
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                    </li>
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                </ul>
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<h5> Why is this page needed? </h5>
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            <div class="item">
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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                <img src="https://static.igem.org/mediawiki/2017/0/06/Npu-banner3.jpg">
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<p>
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            </div>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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                <img src="https://static.igem.org/mediawiki/2017/2/28/Npu-banner2.jpg">
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        <a class="left carousel-control" href="#myCarousel" data-slide="prev">
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        <a class="right carousel-control" href="#myCarousel" data-slide="next">
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            <span class="icon-next"></span>
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        </a>
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        <!-- Controls -->
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    </header>
  
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    <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;">
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        <!-- Page Content -->
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<div class="column half_size">
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<h5> What should this page have?</h5>
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                <div class="col-md-12">
 +
                    <h2 class="page-header" align="center" >Abstract</h2>
 +
                    <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
 +
                        polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 +
                        annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
 +
                        US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which
 +
                        is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution,
 +
                        high energy consumption and it is unsustainable.<br> This year, we aim to use a green and environmentally
 +
                        friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional
 +
                        chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.
 +
                    </h4>
 +
                 
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                </div>
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            </div>
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            <!-- Marketing Icons Section -->
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                <div class="col-md-12">
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                    <h2 class="page-header" align="center">We construct cell factory based on 4 levels, which are—</h2>
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                    <br>
 +
                </div>
 +
            </div>
  
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<ul>
 
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<li>General Support</li>
 
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<li>Project support and advice</li>
 
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<li>Fundraising help and advice</li>
 
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<li>Lab support</li>
 
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<li>Difficult technique support</li>
 
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<li>Project advisor support</li>
 
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<li>Wiki support</li>
 
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<li>Presentation coaching</li>
 
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<li>Human Practices support</li>
 
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
 
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</ul>
 
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</div>
 
  
  
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            <div class="row">
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                    <a href="portfolio-item.html">
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                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png">
 +
                    </a>
 +
                    <h3 align="center">
 +
                        <a href="portfolio-item.html">Core Part</a>
 +
                    </h3>
 +
                    <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type
 +
                        's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic
 +
                        effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design)
 +
                        platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography)
 +
                        and HTS(High throughput screening).
 +
                    </h4>
 +
                </div>
 +
                <div class="col-md-6 img-portfolio">
 +
                    <a href="portfolio-item.html">
 +
                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt="">
 +
                    </a>
 +
                    <h3 align="center">
 +
                        <a href="portfolio-item.html">System</a>
 +
                    </h3>
 +
                    <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient
 +
                        to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms,
 +
                        we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually.
 +
                    </h4>
 +
                </div>
 +
            </div>
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<h5> Can we base our project on a previous one? </h5>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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                <div class="col-md-6 img-portfolio">
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</div>
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                    <a href="portfolio-item.html">
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</div>
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                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/e/ec/Pathway.png" alt="">
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                    </a>
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                    <h3 align="center">
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                        <a href="portfolio-item.html">Pathway</a>
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                    </h3>
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                    <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different
 +
                        optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol
 +
                        metabolism of the two bacteria, so that it could produce the target product acrylic acid using the
 +
                        intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed
 +
                        and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway
 +
                        and reduce the loss of bypass carbon flux.</h4>
 +
                </div>
 +
                <div class="col-md-6 img-portfolio">
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                    <a href="portfolio-item.html">
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                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/6/67/Production2.png" alt="">
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                    </a>
 +
                    <h3 align="center">
 +
                        <a href="portfolio-item.html" >Production</a>
 +
                    </h3>
 +
                    <h4>All of the previous processes are to build the engineering strains which have a high production of acrylic
 +
                        acid that we need. In the subsequent fermentation, we also need to determine the best parameters
 +
                        of the engineering strain.<br> Therefore, we selected the carbon source, Buffer, temperature, pH
 +
                        and other conditions to optimize the cell production process.</h4>
 +
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            </div>
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<h5>Inspiration</h5>
 
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<p>Take a look at what other teams have done:</p>
 
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<ul>
 
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
 
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
 
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
 
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</ul>
 
  
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<h5>Team training and Project start</h5>
 
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
 
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<ul>
 
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
 
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<li>When did you start this course?</li>
 
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<li>Are the syllabus and course materials freely available online?</li>
 
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<li>When did you start your brainstorming?</li>
 
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<li>When did you start in the lab?</li>
 
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<li>When did you start working on  your project?</li>
 
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Revision as of 13:29, 27 October 2017

Abstract

Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution, high energy consumption and it is unsustainable.
This year, we aim to use a green and environmentally friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.

We construct cell factory based on 4 levels, which are—


Core Part

We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type 's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic effect of ceaS2 enzyme.
We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).

System

Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually.

Pathway

We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol metabolism of the two bacteria, so that it could produce the target product acrylic acid using the intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduce the loss of bypass carbon flux.

Production

All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.
Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.