Difference between revisions of "Team:NPU-China/Attributions"

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                 <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China">
 
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                     <li class="active"> <a href="https://2017.igem.org/Team:NPU-China">Home</a> </li>
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                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
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                        <a href="https://2017.igem.org/Team:NPU-China">Home</a>
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                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a>
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                            </li>
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                             <li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a>
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                            </li>
 
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                         </ul>
 
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                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li>
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                             <li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li>
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                            </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a>
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                            </li>
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                             <li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a>
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                            </li>
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                             <li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a>
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                            </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a>
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                            </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a>
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                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a>
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                        <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a>
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                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a>
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                                <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a>
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                     <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li>
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                     <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li>
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                        <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a>
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                     <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li>
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                    </li>
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                     <li>
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                        <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a>
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                    </li>
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                        <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a>
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                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a>
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                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a>
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                                <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a>
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                     <h2 class="page-header" align="center" >Abstract</h2>
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                     <h2 style="text-align:center">Attribution</h2>
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                     <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
+
                   
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                        polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
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                     <h4>Our project is a team-work involving in 13 undergraduates divided into seven groups. Each group was responsible for one aspect such as WIKI construction, art design, wet-lab work, human practices and so on. </h4>
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                        annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
+
                    <h3 style="text-align:center">1.Team set-up and management</h3>
−
                        US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which
+
                    <h4>Zexun Wu set up the NPU-China2016 team and serves as the captain and is in charge of accomplishment of the whole project.<br>
−
                        is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution,
+
                    Yun Liu is responsible for financial affairs.</h4>
−
                        high energy consumption and it is unsustainable.<br> This year, we aim to use a green and environmentally
+
                    <h3 style="text-align:center">2. Wet-lab work</h3>
−
                        friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional
+
                    <h4>Xin Hu, Qi Li, Zexun Wu,Yuan Zhang, Weifeng Lin.<br>
−
                        chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.
+
                    Xinhu and Qi Li are in charge of the plasmid construction, gene transformation and whole cell catalysis. They are also in charge of the gene knockout work in S. cerevisiae. Zexun Wu, Yun Liu, Weifeng Lin are in charge of gene knockout work in E.Coli and have done the work about detection and safety. Xin Hu is the general experiment leader of Wet-lab work too. </h4>
−
                    </h4>
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                    <h3 style="text-align:center">3．Hardware</h3>
−
                 
+
                    <h4>WeiWei Qin is in chare of the design，construction of our hardware and Weifeng Lin helps him do some works at usual.</h4>
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                </div>
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                    <h3 style="text-align:center">4. Art Design</h3>
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            </div>
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                    <h4>Chenyuan Li is responsible for Art Designs including the designs for our team clothing, posters, banner, wiki.</h4>
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                    <h3 style="text-align:center">5. Wiki</h3>
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                    <h4>Yuhan Ke, Zexun Wu, Chenyuan Li worked to accomplish the design and construction of WIKI contents. </h4>
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                    <h3 style="text-align:center">6. Human Practices</h3>
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                     <h2 class="page-header" align="center">We construct cell factory based on 4 levels, which are—</h2>
+
                     <h4>Zexun Wu is responsible for planning and organizing the HP including every social activities. </h4>
 +
                   
 +
                    <h3 style="text-align:center">7. Modeling</h3>
 +
                    <h4>Our metabolic flow model is finished by Weixu Wang in SCAU-China. Yutong Bai is responsible for translating them into English. </h4>
 
                     <br>
 
                     <br>
 +
                    <br>
 +
                   
 +
                   
 +
                    <h2 style="text-align:center">Acknowledgements</h2>
 +
                   
 +
                    <h3 style="text-align:center">1. Special thanks</h3>
 +
                   
 +
                    <h4>We are grateful to Bingzhao Zhuo, who help us set-up a team and knock-out two genes in E.Coli at the beginning of our project. Our project was supported by grants from the <br>
 +
                    Research Center for Ecology and Environmental Sciences of NPU, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Siences, the College of Life Sciences. </h4>
 +
                    <h3 style="text-align:center">2. Lab support</h3>
 +
                   
 +
                    <h4>Our project was mainly conducted in TIB Yeast Genomic Engineering Lab and the lab of Research Center for Ecology and Environmental Sciences of NPU. These two <br>
 +
                   
 +
                    laboratories is a leading research unit focusing on synbio. Therefore, we can successfully carry out our experiments including knock out genes using CRISPR/Cas9 as well as the construction of plasmid and so on. </h4>
 +
                   
 +
                    <h3 style="text-align:center">3. Human practices support</h3>
 +
                   
 +
                    <h4>During the human practices, we received many help and assistances from people and organizations, such as the Xidian University(Holding the forum), Xi’an Aerospace Sunvalor Chemical CO., Ltd, FAFU-China(Holding the CCiC), SCUT-FSE-CHINA(collaborated with us on HP). </h4>
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                    <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type
 
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                        's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic
 
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                        metabolism of the two bacteria, so that it could produce the target product acrylic acid using the
 
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                        intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed
 
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                        and other conditions to optimize the cell production process.</h4>
 
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Revision as of 10:12, 28 October 2017

Attribution

Our project is a team-work involving in 13 undergraduates divided into seven groups. Each group was responsible for one aspect such as WIKI construction, art design, wet-lab work, human practices and so on.

1.Team set-up and management

Zexun Wu set up the NPU-China2016 team and serves as the captain and is in charge of accomplishment of the whole project.
Yun Liu is responsible for financial affairs.

2. Wet-lab work

Xin Hu, Qi Li, Zexun Wu,Yuan Zhang, Weifeng Lin.
Xinhu and Qi Li are in charge of the plasmid construction, gene transformation and whole cell catalysis. They are also in charge of the gene knockout work in S. cerevisiae. Zexun Wu, Yun Liu, Weifeng Lin are in charge of gene knockout work in E.Coli and have done the work about detection and safety. Xin Hu is the general experiment leader of Wet-lab work too.

3．Hardware

WeiWei Qin is in chare of the design，construction of our hardware and Weifeng Lin helps him do some works at usual.

4. Art Design

Chenyuan Li is responsible for Art Designs including the designs for our team clothing, posters, banner, wiki.

5. Wiki

Yuhan Ke, Zexun Wu, Chenyuan Li worked to accomplish the design and construction of WIKI contents.

6. Human Practices

Zexun Wu is responsible for planning and organizing the HP including every social activities.

7. Modeling

Our metabolic flow model is finished by Weixu Wang in SCAU-China. Yutong Bai is responsible for translating them into English.



Acknowledgements

1. Special thanks

We are grateful to Bingzhao Zhuo, who help us set-up a team and knock-out two genes in E.Coli at the beginning of our project. Our project was supported by grants from the
Research Center for Ecology and Environmental Sciences of NPU, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Siences, the College of Life Sciences.

2. Lab support

Our project was mainly conducted in TIB Yeast Genomic Engineering Lab and the lab of Research Center for Ecology and Environmental Sciences of NPU. These two
laboratories is a leading research unit focusing on synbio. Therefore, we can successfully carry out our experiments including knock out genes using CRISPR/Cas9 as well as the construction of plasmid and so on.

3. Human practices support

During the human practices, we received many help and assistances from people and organizations, such as the Xidian University(Holding the forum), Xi’an Aerospace Sunvalor Chemical CO., Ltd, FAFU-China(Holding the CCiC), SCUT-FSE-CHINA(collaborated with us on HP).