Shuimoliuyun (Talk | contribs) |
Shuimoliuyun (Talk | contribs) |
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First, we introduced new pathways into two chassis cells through two or three | First, we introduced new pathways into two chassis cells through two or three | ||
plasmid vectors. | plasmid vectors. | ||
− | pET-28a-ceaS2 | + | <br>pET-28a-ceaS2 |
− | pCDFDuet-gld-DAK | + | <br>pCDFDuet-gld-DAK |
− | pETDuet-NOX-CAT | + | <br>pETDuet-NOX-CAT |
− | YCplac33-LEU-ceaS2 | + | <br>YCplac33-LEU-ceaS2 |
− | YCplac33-LEU-ceaS2-NOX | + | <br>YCplac33-LEU-ceaS2-NOX |
− | YCplac33-URA-gld-DAK | + | <br>YCplac33-URA-gld-DAK |
+ | <br> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2017/c/c7/NPU-image11.png" style="max-width:60%;"></center> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2017/0/00/NPU-image12.png" style="max-width:60%;"></center> | ||
+ | <br> | ||
1:E.gld+DAK;2:S-ceaS2;3,E.NOX-CAT;4.S.NOX-ceaS2;5:DAK;6:NOX;7,ceaS2; | 1:E.gld+DAK;2:S-ceaS2;3,E.NOX-CAT;4.S.NOX-ceaS2;5:DAK;6:NOX;7,ceaS2; | ||
8:gld;9:s.gld-DAK;10:CAT | 8:gld;9:s.gld-DAK;10:CAT | ||
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For E. coli, yields of using new and old synthetic pathways of acrylic acid are as follows: | For E. coli, yields of using new and old synthetic pathways of acrylic acid are as follows: | ||
Conditions: reaction time 42h, PH8.0, glycerol concentration 1% | Conditions: reaction time 42h, PH8.0, glycerol concentration 1% | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <br> |
+ | <img src="https://static.igem.org/mediawiki/2017/b/b5/NPU-image13.png" style="max-width:60%;"><br /> | ||
It can be seen that the acrylic acid yield is increased by 3 times after the introduction | It can be seen that the acrylic acid yield is increased by 3 times after the introduction | ||
of the GlyDH enzyme and the DAK enzyme compared to the introduction of only | of the GlyDH enzyme and the DAK enzyme compared to the introduction of only | ||
Line 265: | Line 270: | ||
indicator | indicator | ||
</br> | </br> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/5/5e/NPU-image14.png" style="max-width:60%;"><br /> |
It can be seen that, similar to the results of E. coli, the introduction of new | It can be seen that, similar to the results of E. coli, the introduction of new | ||
pathways does improve the ability of S. cerevisiae synthesizing acrylic acid. | pathways does improve the ability of S. cerevisiae synthesizing acrylic acid. |
Revision as of 18:46, 1 November 2017