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</h4> | </h4> | ||
<img src="https://static.igem.org/mediawiki/2017/c/c3/NPU-image18.png" style="max-width:60%;"><br /> | <img src="https://static.igem.org/mediawiki/2017/c/c3/NPU-image18.png" style="max-width:60%;"><br /> | ||
− | It can be seen that when the induction temperature was 30 ℃, the enzyme expression and activity were the highest, and the yield of acrylic acid was the best. | + | <h4> |
+ | It can be seen that when the induction temperature was 30 ℃, the enzyme expression and activity were the highest, and the yield of acrylic acid was the best.</h4> | ||
</br> | </br> | ||
+ | <h4> | ||
4.2 the results of production of acrylic acid with different carbon sources | 4.2 the results of production of acrylic acid with different carbon sources | ||
Condition: PH7.4 | Condition: PH7.4 | ||
Reaction time: 16h | Reaction time: 16h | ||
Glucose concentration: 4g/L | Glucose concentration: 4g/L | ||
− | Glycerol concentration: 1%<br /> | + | Glycerol concentration: 1%<br /></h4> |
<img src="https://static.igem.org/mediawiki/2017/8/80/NPU-image19.png" style="max-width:60%;"><br /> | <img src="https://static.igem.org/mediawiki/2017/8/80/NPU-image19.png" style="max-width:60%;"><br /> | ||
− | <h4>It can be seen that the yield of acrylic acid was higher when the glycerol was used | + | <h4> |
+ | It can be seen that the yield of acrylic acid was higher when the glycerol was used | ||
as the carbon source, because the carbon flow rate of the glycerol metabolic | as the carbon source, because the carbon flow rate of the glycerol metabolic | ||
pathway was more concentrated, thus turning more carbon source into acrylic | pathway was more concentrated, thus turning more carbon source into acrylic |
Latest revision as of 02:22, 2 November 2017