Difference between revisions of "Team:NPU-China/Demonstrate"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
 
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                    <li class="active"> <a href="https://2017.igem.org/Team:NPU-China">Home</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li>
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                        </ul>
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                    </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> </li>
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                        </ul>
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                    </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li>
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                        </ul>
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                    </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li>
  
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li>
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                        </ul>
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                    </li>
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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    <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;">
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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                    <h2 class="page-header" align="center" >Abstract</h2>
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                    <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
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                        polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 +
                        annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
 +
                        US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which
 +
                        is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution,
 +
                        high energy consumption and it is unsustainable.<br> This year, we aim to use a green and environmentally
 +
                        friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional
 +
                        chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.
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                    </h4>
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                    <h2 class="page-header" align="center">We construct cell factory based on 4 levels, which are—</h2>
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                    <br>
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<h5> Special track teams </h5>
 
  
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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                    <a href="portfolio-item.html">
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                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png">
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                    </a>
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                    <h3 align="center">
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                        <a href="portfolio-item.html">Core Part</a>
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                    </h3>
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                    <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type
 +
                        's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic
 +
                        effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design)
 +
                        platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography)
 +
                        and HTS(High throughput screening).
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                    </h4>
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                    <a href="portfolio-item.html">
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                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt="">
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                    </a>
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                    <h3 align="center">
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                        <a href="portfolio-item.html">System</a>
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                    </h3>
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                    <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient
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                        to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms,
 +
                        we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually.
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                    </h4>
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                    </a>
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                        <a href="portfolio-item.html">Pathway</a>
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                    </h3>
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                    <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different
 +
                        optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol
 +
                        metabolism of the two bacteria, so that it could produce the target product acrylic acid using the
 +
                        intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed
 +
                        and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway
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                        and reduce the loss of bypass carbon flux.</h4>
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                    </a>
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                    <h3 align="center">
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                        <a href="portfolio-item.html" >Production</a>
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                    </h3>
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                    <h4>All of the previous processes are to build the engineering strains which have a high production of acrylic
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                        acid that we need. In the subsequent fermentation, we also need to determine the best parameters
 +
                        of the engineering strain.<br> Therefore, we selected the carbon source, Buffer, temperature, pH
 +
                        and other conditions to optimize the cell production process.</h4>
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Revision as of 13:30, 27 October 2017

Abstract

Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution, high energy consumption and it is unsustainable.
This year, we aim to use a green and environmentally friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.

We construct cell factory based on 4 levels, which are—


Core Part

We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type 's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic effect of ceaS2 enzyme.
We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).

System

Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually.

Pathway

We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol metabolism of the two bacteria, so that it could produce the target product acrylic acid using the intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduce the loss of bypass carbon flux.

Production

All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.
Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.