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<br><br> | <br><br> | ||
<center><img src="https://static.igem.org/mediawiki/2017/5/59/NPU-formerAA.png" class="img-responsive"></center> | <center><img src="https://static.igem.org/mediawiki/2017/5/59/NPU-formerAA.png" class="img-responsive"></center> | ||
+ | <center><h4>Overview of existing and hypothetical metabolic pathways for biosynthesis of acrylate from sugars. </h4></center> | ||
+ | |||
<br> In the previous experiments, we had further demonstrated the new function of ceaS2 enzyme, which can catalyze the production of acrylic acid with DHAP (hydroxyxy acetone phosphate) or G3P (glyceraldehyde 3-phosphate) as substrate. Because acrylic acid is not the main product of ceaS2 enzyme, the catalytic | <br> In the previous experiments, we had further demonstrated the new function of ceaS2 enzyme, which can catalyze the production of acrylic acid with DHAP (hydroxyxy acetone phosphate) or G3P (glyceraldehyde 3-phosphate) as substrate. Because acrylic acid is not the main product of ceaS2 enzyme, the catalytic | ||
effect of wild-type ceaS2 enzyme is very weak and the yield of acrylic acid is only 1mg / L. So we | effect of wild-type ceaS2 enzyme is very weak and the yield of acrylic acid is only 1mg / L. So we | ||
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<center><img src="https://static.igem.org/mediawiki/2017/8/85/System.png" class="img-responsive"></center> | <center><img src="https://static.igem.org/mediawiki/2017/8/85/System.png" class="img-responsive"></center> | ||
− | + | <center><h4> E.coli - V.S - S.cerevisiae </h4></center> | |
<br><br> The GDC (GlyDH-DAK-ceaS2) pathway was designed in order to improve the ability of chassis cells | <br><br> The GDC (GlyDH-DAK-ceaS2) pathway was designed in order to improve the ability of chassis cells | ||
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<div class="col-md-6"> | <div class="col-md-6"> | ||
<img src="https://static.igem.org/mediawiki/2017/1/10/%E5%A4%A7%E8%82%A0%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2017/1/10/%E5%A4%A7%E8%82%A0%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> | ||
− | + | <center> <h4> the GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) pathway for E.coli </h4></center> | |
</div> | </div> | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
<img src="https://static.igem.org/mediawiki/2017/b/b0/%E9%85%B5%E6%AF%8D%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2017/b/b0/%E9%85%B5%E6%AF%8D%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> | ||
− | + | <center> <h4> the GNDC(GlyDH-NOX-DAK-ceaS2) pathway for S.cerevisiae </h4></center> | |
</div> | </div> | ||
</div> | </div> | ||
− | + | </h4> | |
+ | <h4> | ||
<br><br> We used whole cell catalysis to carry out the initial acrylic acid synthesis "fermentation" process. | <br><br> We used whole cell catalysis to carry out the initial acrylic acid synthesis "fermentation" process. | ||
The advantage of whole cell catalysis is that the intracellular complete multi-enzyme system can | The advantage of whole cell catalysis is that the intracellular complete multi-enzyme system can |
Revision as of 21:24, 1 November 2017