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</h4> | </h4> | ||
【ceaS2酶结构图+5埃范围内活性中心示意图】 | 【ceaS2酶结构图+5埃范围内活性中心示意图】 | ||
+ | <div id="PATHWAY" style="padding-top:1000px;margin-top:-1000px;"> | ||
+ | <h2 style="text-align:center" >PATHWAY</h2> | ||
+ | <h4>The carbon flow rate of the glycerol metabolic pathway is low. In order to solve the problem, we need | ||
+ | reconstruction and optimization of the original metabolic pathway. | ||
+ | <br> | ||
+ | <br> RE-Construction:We designed the GDC (GlyDH-DAK-Ceas2) pathway to produce acrylic acid from glycerol. | ||
+ | In this pathway, GlyDH(Glycerol dehydrogenase) can efficiently convert Glycerol into DHA(1,3-Dihydroxyacetone). | ||
+ | Then DAK (Dihydroxyacetone kinase) converts DHA into DHAP. Finally, ceaS2 converts DHAP into acrylic | ||
+ | acid. In addition, because GlyDH depends on NAD+, we added two reduction models, NOX (NADH dehydrogenase | ||
+ | )and CAT(Catalase), to the pathway, with the purpose of providing the required reduction force for | ||
+ | GLY DH through the two layers of substrate level cycle. At last, we construct a new pathway for acrylic | ||
+ | acid synthesis- GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) | ||
+ | <br> The genes of GlyDH and DAK were constructed on two MCS (multiple cloning sites) on the backbone | ||
+ | of pCDFDuet-1 plasmid. NOX and CAT were constructed on two MCSs on the backbone of pETDuet-1 plasmid. | ||
+ | ) (质粒图注释) | ||
+ | <br> | ||
+ | </h4> | ||
+ | 【E.coli新路径图(含旧路径部分),区别主要途径和还原力模块+质粒图】 | ||
+ | </div> | ||
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<h2 style="text-align:center">SYSTEM</h2> | <h2 style="text-align:center">SYSTEM</h2> |
Revision as of 15:19, 30 October 2017