Shuimoliuyun (Talk | contribs) |
Shuimoliuyun (Talk | contribs) |
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for S.cerevisiae. | for S.cerevisiae. | ||
<br><br> 【新途径+质粒图】 The genes of GlyDH and DAK were constructed on the backbone of YCPlac33 plasmid with | <br><br> 【新途径+质粒图】 The genes of GlyDH and DAK were constructed on the backbone of YCPlac33 plasmid with | ||
− | URA marker. We used the ADH1 promoter and | + | URA marker. We used the ADH1 promoter and tGPD1 terminator for GlyDH, the PGK1 promoter and the |
tPFK1 terminator for DAK. NOX and ceaS2 were constructed on the backbone of the other YCPlac33 | tPFK1 terminator for DAK. NOX and ceaS2 were constructed on the backbone of the other YCPlac33 | ||
− | plasmid. We replaced URA marker with | + | plasmid. We replaced URA marker with Leu marker to screen for two plasmids easily. We used the |
TEF2 promoter and tRPS2 terminator for GlyDH, the same promoter and terminator as the original | TEF2 promoter and tRPS2 terminator for GlyDH, the same promoter and terminator as the original | ||
pathway for ceaS2. (质粒图注释) | pathway for ceaS2. (质粒图注释) |
Revision as of 14:40, 1 November 2017