Shuimoliuyun (Talk | contribs) |
Shuimoliuyun (Talk | contribs) |
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of 573 amino acids. We need to use bioinformatics to analyze and simulate, in order to help us | of 573 amino acids. We need to use bioinformatics to analyze and simulate, in order to help us | ||
decide the correct proposal. | decide the correct proposal. | ||
− | <br><br> We constructed ceaS2 enzyme mutants using the AEMD (Auto Enzyme Mutation Design) platform. We | + | <br> |
+ | <center><img src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png" class="img-responsive"></center> | ||
+ | <br> We constructed ceaS2 enzyme mutants using the AEMD (Auto Enzyme Mutation Design) platform. We | ||
constructed the ceaS2 wild-type sequence on pET-28a plasmid. We used pET-28a-ceaS2 plasmid as | constructed the ceaS2 wild-type sequence on pET-28a plasmid. We used pET-28a-ceaS2 plasmid as | ||
a template to create point mutation, and then transformed the plasmid into BL21. Then, we did | a template to create point mutation, and then transformed the plasmid into BL21. Then, we did | ||
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</div> | </div> | ||
【ceaS2酶结构图+5埃范围内活性中心示意图】 | 【ceaS2酶结构图+5埃范围内活性中心示意图】 | ||
− | + | ||
<div class="container" style="padding-top:50px"> | <div class="container" style="padding-top:50px"> |
Revision as of 16:24, 1 November 2017