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reduction force for GLY DH through the two layers of substrate level cycle. At last, we construct | reduction force for GLY DH through the two layers of substrate level cycle. At last, we construct | ||
a new pathway for acrylic acid synthesis- GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) | a new pathway for acrylic acid synthesis- GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) | ||
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+ | <img src="https://static.igem.org/mediawiki/2017/5/5c/%E5%A4%A7%E8%82%A01_pCDFDuet-gld-DAK_6550.png" class="img-responsive"> | ||
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<br><br> The genes of GlyDH and DAK were constructed on two MCS (multiple cloning sites) on the backbone | <br><br> The genes of GlyDH and DAK were constructed on two MCS (multiple cloning sites) on the backbone | ||
of pCDFDuet-1 plasmid. NOX and CAT were constructed on two MCSs on the backbone of pETDuet-1 | of pCDFDuet-1 plasmid. NOX and CAT were constructed on two MCSs on the backbone of pETDuet-1 | ||
− | plasmid. | + | plasmid. |
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to GAACF1.0, we used the YCPlac33 plasmid with URA defect screening marker as the backbone and | to GAACF1.0, we used the YCPlac33 plasmid with URA defect screening marker as the backbone and | ||
used the pTDH3 constitutive promoter and tPFK1 constitutive terminator to construct ceaS2 plasmid. | used the pTDH3 constitutive promoter and tPFK1 constitutive terminator to construct ceaS2 plasmid. | ||
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+ | <h4> </h4> | ||
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+ | <img src="https://static.igem.org/mediawiki/2017/7/70/%E5%A4%A7%E8%82%A03_pETDuet-NOX-CAT_7451.png" class="img-responsive"> | ||
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<br><br> 【S.C图+路径图+质粒图】 We confirmed the proposal can make S.cerevisiae produce acrylic acid, but the | <br><br> 【S.C图+路径图+质粒图】 We confirmed the proposal can make S.cerevisiae produce acrylic acid, but the | ||
yield is low, so we decided to optimize it. | yield is low, so we decided to optimize it. |
Revision as of 18:21, 1 November 2017