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reduction force for GLY DH through the two layers of substrate level cycle. At last, we construct | reduction force for GLY DH through the two layers of substrate level cycle. At last, we construct | ||
a new pathway for acrylic acid synthesis- GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) | a new pathway for acrylic acid synthesis- GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) | ||
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+ | <center><img src="https://static.igem.org/mediawiki/2017/1/10/%E5%A4%A7%E8%82%A0%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"></center> | ||
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<br><br> Therefore, in the choice of the chassis organism, we tested two organisms, E. coli MG1655 and | <br><br> Therefore, in the choice of the chassis organism, we tested two organisms, E. coli MG1655 and | ||
Saccharomyces cerevisiae BY4741. BY4741 has a great ability to metabolize glycerol. According | Saccharomyces cerevisiae BY4741. BY4741 has a great ability to metabolize glycerol. According | ||
− | to GAACF1.0, we used the YCPlac33 plasmid with | + | to GAACF1.0, we used the YCPlac33 plasmid with LEU defect screening marker as the backbone and |
used the pTDH3 constitutive promoter and tPFK1 constitutive terminator to construct ceaS2 plasmid. | used the pTDH3 constitutive promoter and tPFK1 constitutive terminator to construct ceaS2 plasmid. | ||
Revision as of 18:29, 1 November 2017