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Saccharomyces cerevisiae BY4741. BY4741 has a great ability to metabolize glycerol. According | Saccharomyces cerevisiae BY4741. BY4741 has a great ability to metabolize glycerol. According | ||
to GAACF1.0, we used the YCPlac33 plasmid with LEU defect screening marker as the backbone and | to GAACF1.0, we used the YCPlac33 plasmid with LEU defect screening marker as the backbone and | ||
− | used the pTDH3 constitutive promoter and tPFK1 constitutive terminator to construct ceaS2 plasmid. | + | used the pTDH3 constitutive promoter and tPFK1 constitutive terminator to construct ceaS2 plasmid.<br> |
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+ | <center><img src="https://static.igem.org/mediawiki/2017/1/15/NPU-%E9%85%B5%E6%AF%8D.jpg" class="img-responsive"></center> | ||
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<div class="col-md-12" style="padding-top:30px"> | <div class="col-md-12" style="padding-top:30px"> | ||
− | <div class="col-md- | + | <div class="col-md-6"> |
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<img src="https://static.igem.org/mediawiki/2017/7/7b/%E9%85%B5%E6%AF%8D%E5%8E%9F%E5%A7%8B%E4%BB%A3%E8%B0%A2%E5%9B%BE.png" class="img-responsive"> | <img src="https://static.igem.org/mediawiki/2017/7/7b/%E9%85%B5%E6%AF%8D%E5%8E%9F%E5%A7%8B%E4%BB%A3%E8%B0%A2%E5%9B%BE.png" class="img-responsive"> | ||
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</div> | </div> | ||
− | <div class="col-md- | + | <div class="col-md-6"> |
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/b/b0/%E9%85%B5%E6%AF%8D%E8%B7%AF%E5%BE%84%E5%9B%BE.png" class="img-responsive"> |
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</div> | </div> | ||
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</div> | </div> | ||
− | + | <br> We confirmed the proposal can make S.cerevisiae produce acrylic acid, but the | |
yield is low, so we decided to optimize it. | yield is low, so we decided to optimize it. | ||
<br> First, according to GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) in E.coli, we added NOX to the pathway(the | <br> First, according to GNCDC(GlyDH-NOX-CAT-DAK-ceaS2) in E.coli, we added NOX to the pathway(the | ||
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</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
− | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/c/cd/%E9%85%B5%E6%AF%8D4_Y181-Leu-ceas2_8558.png" class="img-responsive"> |
<h4> </h4> | <h4> </h4> | ||
</div> | </div> |
Revision as of 18:57, 1 November 2017