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<a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> | <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> | ||
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+ | <h4>The theme of our project this year is Manufacture, so we have been trying to contrive a series of hardwares | ||
+ | to model the industrial production and enhance our method. However, the gap does exist between the | ||
+ | reality and our expectation. | ||
+ | </h4> | ||
− | < | + | <h3 style="text-align:center">List of what we use</h3> |
− | <h4> | + | <h4>By listing the main items we have purchased and actually used, we hope to provide some experience and |
− | the | + | inspiration for the future teams wishing to have HW productions. |
− | + | </h4> | |
− | + | <div align="center"> | |
− | + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/1/19/Hardware_tab.png" width="60%" height="60%"> | |
+ | </div> | ||
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− | < | + | <h3 style="text-align:center">Hardware Design</h3> |
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+ | <h4>Our system is mainly composed of two parts: reaction part and concentrated crystallization part. The | ||
+ | overall idea is that the reaction part provides the bacteria with appropriate reaction temperature, | ||
+ | adequate oxygen and the prevention of bacterial precipitation. After a certain period of time, the | ||
+ | reaction solution will be transferred to the concentrated crystallization part, where the concentration | ||
+ | of the production is increased by the reverse osmosis membrane; the temperature of the reaction | ||
+ | solution is lowered by the device, and the acrylic acid is finally crystallized. | ||
+ | </h4> | ||
− | < | + | <h4>1)Reaction Part</h4> |
− | + | ||
− | <h4> | + | <h4>The reaction part mainly uses miniature DC self-priming pump, foam machine, acrylic material container, |
− | + | aluminum hollow cylinder (built-in heating wire) and NTC thermistor temperature sensor. | |
+ | </h4> | ||
+ | </div> | ||
+ | <div class="col-md-12" style="padding-top:30px"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d0/%E5%B0%8F_Fig3Miniature_DC_self_-_priming_pump_.jpg" class="img-responsive"> | ||
+ | <h4>Fig1 Miniature DC self - priming pump</h4> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/4a/%E5%B0%8F_Hollow_cylinder_made_of_aluminum_alloy_.jpg" class="img-responsive"> | ||
+ | <h4>Fig2 Hollow cylinder made of aluminum alloy</h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-12" style="padding-top:30px"> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/e6/%E5%B0%8F_Hontainer_made_of_Acrylics.jpg" class="img-responsive"> | ||
+ | <h4>Fig3 Container made of Acrylics</h4> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f4/%E5%8F%8D%E5%BA%94%E9%83%A8%E6%BC%94%E7%A4%BA.gif" class="img-responsive" | ||
+ | width="450" height="450"> | ||
+ | <h4>Fig4 3D design drawing Booster pump</h4> | ||
+ | </div> | ||
+ | </div> | ||
− | + | <div class="col-md-12"> | |
+ | |||
+ | <h4>Instruction:When the system starts, the pump will continue to pump the upper reaction solution, which | ||
+ | will blend a large number of air bubbles after passing through the foam machine, and then will be pumped | ||
+ | back from the bottom reaction container. Furthermore, since the reaction vessel is cylindrical and | ||
+ | the bubbles are continuously rising, the purpose of stirring the reaction solution and providing | ||
+ | sufficient air can be achieved. Simultaneously, with the help of the temperature sensor, the system | ||
+ | will be able to control heating wire inside the aluminum alloy cylinder, so that the reaction solution | ||
+ | can maintain a suitable temperature range. Also, the poor thermal conductivity of acrylic materials | ||
+ | is conducive to insulation. (We had considered heating the air mixed with the reaction solution at | ||
+ | the foaming machine so that the reaction solution pumped back to the reaction vessel would be blended | ||
+ | with a large amount of hot air, which could replace the additional heating device, but due to the | ||
+ | safety and energy efficiency, this thought was given up.) | ||
+ | </h4> | ||
+ | <h4>2)Concentrated Crystallization Part</h4> | ||
− | <h4> | + | <h4>After the reaction has been performed for one cycle, the reaction part stops, and the reaction solution |
− | + | is thus introduced into the concentrated crystallization part and the work begins. | |
− | + | <br> In this part we mainly use the acrylic material container, aluminum alloy cold source (including | |
+ | collection function), reverse osmosis membrane, pressure pump, temperature sensor, water level sensor | ||
+ | and semiconductor cooling film, etc. | ||
</h4> | </h4> | ||
+ | </div> | ||
− | + | <div class="col-md-12" style="padding-top:30px"> | |
− | </ | + | <div class="col-md-6"> |
+ | <img src="https://static.igem.org/mediawiki/2017/7/75/%E5%B0%8F_Hontainer_made_of_Acrylics_.jpg" class="img-responsive"> | ||
+ | <h4>Fig5 Container made of Acrylics</h4> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/3/36/%E5%B0%8F_Cold_source_produced_by_aluminum_alloy_.jpg" class="img-responsive"> | ||
+ | <h4>Fig6 Cold source produced by aluminum alloy</h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-12" style="padding-top:30px"> | ||
+ | <div class="col-md-6"> | ||
− | + | <img src="https://static.igem.org/mediawiki/2017/e/ec/%E5%B0%8F_Reverse_osmosis_RO_membrane_and_semiconductor.jpg" class="img-responsive"> | |
− | + | <h4>Fig7 Reverse osmosis RO membrane and<br>semiconductor refrigeration chip</h4> | |
− | + | </div> | |
− | + | <div class="col-md-6"> | |
− | + | <img src="https://static.igem.org/mediawiki/2017/8/8e/%E5%B0%8F_Booster_pump.jpg" class="img-responsive"> | |
− | + | <h4>Fig8 Booster pump</h4> | |
− | + | </div> | |
− | + | </div> | |
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− | < | + | <img src="https://static.igem.org/mediawiki/2017/7/7f/%E6%B5%93%E7%BC%A9%E9%83%A8.gif" class="img-responsive"> |
− | + | <h4>Fig9 3D design drawing</h4> | |
− | + | </div> | |
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− | </h4> | + | |
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+ | <div class="col-md-12"> | ||
+ | <h4>Instruction:Concentrated crystallization part is relatively independent. As long as the system detects | ||
+ | that the internal water level is higher than the reverse osmosis membrane, the part will start to | ||
+ | work. With the pressure pump and reverse osmosis membrane, the water of the reaction solution will | ||
+ | be continuously extracted, which continues to improve the concentration of acrylic acid. And the | ||
+ | semiconductor cooling film will help keep the solution temperature below the melting point | ||
+ | of acrylic acid, which is 286K. After many rounds of cycles, acrylic acid crystals can be collected on the | ||
+ | bottom cooling source (but mixed with engineered bacteria).</h4> | ||
+ | <h4> 3)System Controlling</h4> | ||
+ | <div class="col-md-12" style="padding-top:10px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/c/c0/NPU-HW-%E7%94%B5%E8%B7%AF%E5%9B%BE.jpg" class="img-responsive"> | ||
+ | <h4> | ||
+ | <br> In order to keep as much as possible in line with the actual industrial production, our device involves | ||
+ | a lot of electronic components, pumps, etc., so we designed a set of controlling procedures. | ||
+ | <br> For the sake of our lack of experience and staff, we chose to master the Arduino controlling board due | ||
+ | to its lower difficulty, which shortened our production time. In addition, we also use 1602LCD display, | ||
+ | Relay module, NTC thermistor temperature sensor, adjustable DC power supply, semiconductor cooling | ||
+ | film, arduino controlling panel, water level sensor, DuPont lines, LCD1602 LCD adapter board, Motor | ||
+ | drive extension and so on. | ||
+ | <br> Here is our main program of the controlling system:<a href="https://static.igem.org/mediawiki/2017/4/48/Main_program_of_the_controlling_system.docx">(DOWNLOAD HERE!)</a> | ||
+ | </h4> | ||
+ | <h3 style="text-align:center">Video</h3> | ||
+ | <div align="center"> | ||
+ | <video src="https://static.igem.org/mediawiki/2017/4/45/NPU-china-hardware.mp4" controls="controls"></video> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <h3 style="text-align:center">Impression summary</h3> | ||
+ | |||
+ | <h4>Besides no relevant laboratory and company support, our staff is also scarce (the main staff is only | ||
+ | one person). We had a difficult time accomplishing our initial HW work. So in this part, we hope to be able | ||
+ | to provide a profound lesson. | ||
+ | <br> Before the actual production and debugging, full consideration and deduction must be carried out. | ||
+ | The delivery of the things bought often cost two or three days (In China, we mainly shop online, | ||
+ | so the delivery time needs to be considered), and the purchase of goods may be inconsistent with what | ||
+ | we wanted. Plus, the negligence of some important details may also lead to the purchase of wrong things, | ||
+ | which will waste a lot of valuable time. | ||
+ | <br> If you want to know more or cooperate with us, welcome to contact us. | ||
+ | </h4> | ||
</div> | </div> |
Latest revision as of 19:27, 1 November 2017