Difference between revisions of "Team:NPU-China/Hardware"

 
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                     <h4>The theme of our project this year is Manufacture, so we have been trying to contrive a series of hardware
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                     <h4>The theme of our project this year is Manufacture, so we have been trying to contrive a series of hardwares
 
                         to model the industrial production and enhance our method. However, the gap does exist between the
 
                         to model the industrial production and enhance our method. However, the gap does exist between the
 
                         reality and our expectation.
 
                         reality and our expectation.
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                     <h4>By listing the main items we have purchased and actually used, we hope to provide some experience and
 
                     <h4>By listing the main items we have purchased and actually used, we hope to provide some experience and
                         inspiration for the future team wishing to have HW production.
+
                         inspiration for the future teams wishing to have HW productions.
 
                     </h4>
 
                     </h4>
 
                     <div align="center">
 
                     <div align="center">
                            <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/1/19/Hardware_tab.png" width="60%" height="60%" >
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                        <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/1/19/Hardware_tab.png" width="60%" height="60%">
 
                     </div>
 
                     </div>
                   
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                         adequate oxygen and the prevention of bacterial precipitation. After a certain period of time, the
 
                         adequate oxygen and the prevention of bacterial precipitation. After a certain period of time, the
 
                         reaction solution will be transferred to the concentrated crystallization part, where the concentration
 
                         reaction solution will be transferred to the concentrated crystallization part, where the concentration
                         of the productivity is increased by the reverse osmosis membrane; the temperature of the reaction
+
                         of the production is increased by the reverse osmosis membrane; the temperature of the reaction
 
                         solution is lowered by the device, and the acrylic acid is finally crystallized.
 
                         solution is lowered by the device, and the acrylic acid is finally crystallized.
 
                     </h4>
 
                     </h4>
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                         aluminum hollow cylinder (built-in heating wire) and NTC thermistor temperature sensor.
 
                         aluminum hollow cylinder (built-in heating wire) and NTC thermistor temperature sensor.
 
                     </h4>
 
                     </h4>
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                </div>
 +
                <div class="col-md-12" style="padding-top:30px">
 +
                    <div class="col-md-6">
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                        <img src="https://static.igem.org/mediawiki/2017/d/d0/%E5%B0%8F_Fig3Miniature_DC_self_-_priming_pump_.jpg" class="img-responsive">
 +
                        <h4>Fig1 Miniature DC self - priming pump</h4>
 +
                    </div>
 +
                    <div class="col-md-6">
 +
                        <img src="https://static.igem.org/mediawiki/2017/4/4a/%E5%B0%8F_Hollow_cylinder_made_of_aluminum_alloy_.jpg" class="img-responsive">
 +
                        <h4>Fig2 Hollow cylinder made of aluminum alloy</h4>
 +
                    </div>
 +
                </div>
 +
                <div class="col-md-12" style="padding-top:30px">
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                    <div class="col-md-6">
  
                    <h4>
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                        <img src="https://static.igem.org/mediawiki/2017/e/e6/%E5%B0%8F_Hontainer_made_of_Acrylics.jpg" class="img-responsive">
                         (图)Fig1 Hontainer made of Acrylics Fig2 Hollow cylinder made of aluminum alloy Fig3Miniature DC self - priming pump Fig4
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                         <h4>Fig3 Container made of Acrylics</h4>
                         3D design drawing
+
                    </div>
                     </h4>
+
                    <div class="col-md-6">
 +
                         <img src="https://static.igem.org/mediawiki/2017/f/f4/%E5%8F%8D%E5%BA%94%E9%83%A8%E6%BC%94%E7%A4%BA.gif" class="img-responsive"
 +
                            width="450" height="450">
 +
                        <h4>Fig4 3D design drawing Booster pump</h4>
 +
                     </div>
 +
                </div>
  
 +
                <div class="col-md-12">
  
                     <h4>Instructions:When the system starts, the pump will continue to pump the upper reaction solution, which
+
                     <h4>Instruction:When the system starts, the pump will continue to pump the upper reaction solution, which
                         will blend a large amount of air bubbles after passing the foam machine, and then will be pumped
+
                         will blend a large number of air bubbles after passing through the foam machine, and then will be pumped
 
                         back from the bottom reaction container. Furthermore, since the reaction vessel is cylindrical and
 
                         back from the bottom reaction container. Furthermore, since the reaction vessel is cylindrical and
 
                         the bubbles are continuously rising, the purpose of stirring the reaction solution and providing
 
                         the bubbles are continuously rising, the purpose of stirring the reaction solution and providing
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                         can maintain a suitable temperature range. Also, the poor thermal conductivity of acrylic materials
 
                         can maintain a suitable temperature range. Also, the poor thermal conductivity of acrylic materials
 
                         is conducive to insulation. (We had considered heating the air mixed with the reaction solution at
 
                         is conducive to insulation. (We had considered heating the air mixed with the reaction solution at
                         the foaming machine so that the reaction liquid pumped back to the reaction vessel would be blended
+
                         the foaming machine so that the reaction solution pumped back to the reaction vessel would be blended
 
                         with a large amount of hot air, which could replace the additional heating device, but due to the
 
                         with a large amount of hot air, which could replace the additional heating device, but due to the
 
                         safety and energy efficiency, this thought was given up.)
 
                         safety and energy efficiency, this thought was given up.)
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                         <br> In this part we mainly use the acrylic material container, aluminum alloy cold source (including
 
                         <br> In this part we mainly use the acrylic material container, aluminum alloy cold source (including
 
                         collection function), reverse osmosis membrane, pressure pump, temperature sensor, water level sensor
 
                         collection function), reverse osmosis membrane, pressure pump, temperature sensor, water level sensor
                         and semiconductor refrigeration film, etc.
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                         and semiconductor cooling film, etc.
 
                     </h4>
 
                     </h4>
 +
                </div>
  
                    <h4>
 
                        (图)Fig5 Hontainer made of Acrylics Fig6 Cold source produced by aluminum alloy Fig 7 Reverse osmosis RO membrane Fig9 3D
 
                        design drawing
 
                    </h4>
 
  
 +
                <div class="col-md-12" style="padding-top:30px">
 +
                    <div class="col-md-6">
 +
                        <img src="https://static.igem.org/mediawiki/2017/7/75/%E5%B0%8F_Hontainer_made_of_Acrylics_.jpg" class="img-responsive">
 +
                        <h4>Fig5 Container made of Acrylics</h4>
 +
                    </div>
 +
                    <div class="col-md-6">
 +
                        <img src="https://static.igem.org/mediawiki/2017/3/36/%E5%B0%8F_Cold_source_produced_by_aluminum_alloy_.jpg" class="img-responsive">
 +
                        <h4>Fig6 Cold source produced by aluminum alloy</h4>
 +
                    </div>
 +
                </div>
 +
                <div class="col-md-12" style="padding-top:30px">
 +
                    <div class="col-md-6">
  
                     <h4>Instructions:Concentrated crystallization part is relatively independent. As long as the system detects
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                        <img src="https://static.igem.org/mediawiki/2017/e/ec/%E5%B0%8F_Reverse_osmosis_RO_membrane_and_semiconductor.jpg" class="img-responsive">
 +
                        <h4>Fig7 Reverse osmosis RO membrane and<br>semiconductor refrigeration chip</h4>
 +
                    </div>
 +
                    <div class="col-md-6">
 +
                        <img src="https://static.igem.org/mediawiki/2017/8/8e/%E5%B0%8F_Booster_pump.jpg" class="img-responsive">
 +
                        <h4>Fig8 Booster pump</h4>
 +
                    </div>
 +
                </div>
 +
                <div class="col-md-12" style="padding-top:30px" align="center">
 +
 
 +
                    <img src="https://static.igem.org/mediawiki/2017/7/7f/%E6%B5%93%E7%BC%A9%E9%83%A8.gif" class="img-responsive">
 +
                    <h4>Fig9 3D design drawing</h4>
 +
                </div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
                <div class="col-md-12">
 +
                     <h4>Instruction:Concentrated crystallization part is relatively independent. As long as the system detects
 
                         that the internal water level is higher than the reverse osmosis membrane, the part will start to
 
                         that the internal water level is higher than the reverse osmosis membrane, the part will start to
 
                         work. With the pressure pump and reverse osmosis membrane, the water of the reaction solution will
 
                         work. With the pressure pump and reverse osmosis membrane, the water of the reaction solution will
 
                         be continuously extracted, which continues to improve the concentration of acrylic acid. And the
 
                         be continuously extracted, which continues to improve the concentration of acrylic acid. And the
                         semiconductor refrigeration film will help keep the solution temperature below the melting point
+
                         semiconductor cooling film will help keep the solution temperature below the melting point
                         286K of acrylic acid. After many rounds of cycles, acrylic acid crystals can be collected on the
+
                         of acrylic acid, which is 286K. After many rounds of cycles, acrylic acid crystals can be collected on the
                         bottom cooling source (but mixed with engineered bacteria).
+
                         bottom cooling source (but mixed with engineered bacteria).</h4>
                        <br> 3)System Controlling
+
                    <h4> 3)System Controlling</h4>
                        <br> In order to keep as much as possible in line with the actual industrial production, our device involves
+
                  <div class="col-md-12" style="padding-top:10px">
 +
 
 +
                <img src="https://static.igem.org/mediawiki/2017/c/c0/NPU-HW-%E7%94%B5%E8%B7%AF%E5%9B%BE.jpg" class="img-responsive">
 +
 
 +
 
 +
                    <h4>
 +
                      <br> In order to keep as much as possible in line with the actual industrial production, our device involves
 
                         a lot of electronic components, pumps, etc., so we designed a set of controlling procedures.
 
                         a lot of electronic components, pumps, etc., so we designed a set of controlling procedures.
                         <br> Because of our lack of experience and staff, we chose to master the Arduino controlling board due
+
                         <br> For the sake of our lack of experience and staff, we chose to master the Arduino controlling board due
 
                         to its lower difficulty, which shortened our production time. In addition, we also use 1602LCD display,
 
                         to its lower difficulty, which shortened our production time. In addition, we also use 1602LCD display,
                         Relay module, NTC thermistor temperature sensor, Adjustable DC power supply, Semiconductor refrigeration
+
                         Relay module, NTC thermistor temperature sensor, adjustable DC power supply, semiconductor cooling
                         chip, Arduino controlling panel, Water level sensor, DuPont line, LCD1602 LCD adapter board, Motor
+
                         film, arduino controlling panel, water level sensor, DuPont lines, LCD1602 LCD adapter board, Motor
 
                         drive extension and so on.
 
                         drive extension and so on.
                         <br> Here is our main program of the controlling system:
+
                         <br> Here is our main program of the controlling system:<a href="https://static.igem.org/mediawiki/2017/4/48/Main_program_of_the_controlling_system.docx">(DOWNLOAD HERE!)</a>
 
                     </h4>
 
                     </h4>
  
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                     <h3 style="text-align:center">Video</h3>
 
                     <h3 style="text-align:center">Video</h3>
                     <video src="https://static.igem.org/mediawiki/2017/4/45/NPU-china-hardware.mp4" controls="controls" align="center"></video>
+
                     <div align="center">
 +
                        <video src="https://static.igem.org/mediawiki/2017/4/45/NPU-china-hardware.mp4" controls="controls"></video>
 +
                    </div>
 +
 
  
  
 
                     <h3 style="text-align:center">Impression summary</h3>
 
                     <h3 style="text-align:center">Impression summary</h3>
  
                     <h4>We have no relevant laboratory and company support, staff is also very scarce (the main staff is only
+
                     <h4>Besides no relevant laboratory and company support, our staff is also scarce (the main staff is only
                         one person). The road of our first HW work was quite stumbling. So in this part, we hope to be able
+
                         one person). We had a difficult time accomplishing our initial HW work. So in this part, we hope to be able
                         to provide a lesson that feels most profound:
+
                         to provide a profound lesson.
 
                         <br> Before the actual production and debugging, full consideration and deduction must be carried out.
 
                         <br> Before the actual production and debugging, full consideration and deduction must be carried out.
                         The delivery of the things bought often cost two or three days (in China, we mainly do online shopping,
+
                         The delivery of the things bought often cost two or three days (In China, we mainly shop online,
                         so the courier time needs to be considered), and the purchase of goods may be inconsistent with what
+
                         so the delivery time needs to be considered), and the purchase of goods may be inconsistent with what
                         we wanted. Plus, the negligence of some important details may lead to the purchase of wrong things,
+
                         we wanted. Plus, the negligence of some important details may also lead to the purchase of wrong things,
 
                         which will waste a lot of valuable time.
 
                         which will waste a lot of valuable time.
                         <br> If you want to know more or cooperate with us, welcome to contact us
+
                         <br> If you want to know more or cooperate with us, welcome to contact us.
 
                     </h4>
 
                     </h4>
  

Latest revision as of 19:27, 1 November 2017

The theme of our project this year is Manufacture, so we have been trying to contrive a series of hardwares to model the industrial production and enhance our method. However, the gap does exist between the reality and our expectation.

List of what we use

By listing the main items we have purchased and actually used, we hope to provide some experience and inspiration for the future teams wishing to have HW productions.

Hardware Design

Our system is mainly composed of two parts: reaction part and concentrated crystallization part. The overall idea is that the reaction part provides the bacteria with appropriate reaction temperature, adequate oxygen and the prevention of bacterial precipitation. After a certain period of time, the reaction solution will be transferred to the concentrated crystallization part, where the concentration of the production is increased by the reverse osmosis membrane; the temperature of the reaction solution is lowered by the device, and the acrylic acid is finally crystallized.

1)Reaction Part

The reaction part mainly uses miniature DC self-priming pump, foam machine, acrylic material container, aluminum hollow cylinder (built-in heating wire) and NTC thermistor temperature sensor.

Fig1 Miniature DC self - priming pump

Fig2 Hollow cylinder made of aluminum alloy

Fig3 Container made of Acrylics

Fig4 3D design drawing Booster pump

Instruction:When the system starts, the pump will continue to pump the upper reaction solution, which will blend a large number of air bubbles after passing through the foam machine, and then will be pumped back from the bottom reaction container. Furthermore, since the reaction vessel is cylindrical and the bubbles are continuously rising, the purpose of stirring the reaction solution and providing sufficient air can be achieved. Simultaneously, with the help of the temperature sensor, the system will be able to control heating wire inside the aluminum alloy cylinder, so that the reaction solution can maintain a suitable temperature range. Also, the poor thermal conductivity of acrylic materials is conducive to insulation. (We had considered heating the air mixed with the reaction solution at the foaming machine so that the reaction solution pumped back to the reaction vessel would be blended with a large amount of hot air, which could replace the additional heating device, but due to the safety and energy efficiency, this thought was given up.)

2)Concentrated Crystallization Part

After the reaction has been performed for one cycle, the reaction part stops, and the reaction solution is thus introduced into the concentrated crystallization part and the work begins.
In this part we mainly use the acrylic material container, aluminum alloy cold source (including collection function), reverse osmosis membrane, pressure pump, temperature sensor, water level sensor and semiconductor cooling film, etc.

Fig5 Container made of Acrylics

Fig6 Cold source produced by aluminum alloy

Fig7 Reverse osmosis RO membrane and
semiconductor refrigeration chip

Fig8 Booster pump

Fig9 3D design drawing

Instruction:Concentrated crystallization part is relatively independent. As long as the system detects that the internal water level is higher than the reverse osmosis membrane, the part will start to work. With the pressure pump and reverse osmosis membrane, the water of the reaction solution will be continuously extracted, which continues to improve the concentration of acrylic acid. And the semiconductor cooling film will help keep the solution temperature below the melting point of acrylic acid, which is 286K. After many rounds of cycles, acrylic acid crystals can be collected on the bottom cooling source (but mixed with engineered bacteria).

3)System Controlling


In order to keep as much as possible in line with the actual industrial production, our device involves a lot of electronic components, pumps, etc., so we designed a set of controlling procedures.
For the sake of our lack of experience and staff, we chose to master the Arduino controlling board due to its lower difficulty, which shortened our production time. In addition, we also use 1602LCD display, Relay module, NTC thermistor temperature sensor, adjustable DC power supply, semiconductor cooling film, arduino controlling panel, water level sensor, DuPont lines, LCD1602 LCD adapter board, Motor drive extension and so on.
Here is our main program of the controlling system:(DOWNLOAD HERE!)

Video

Impression summary

Besides no relevant laboratory and company support, our staff is also scarce (the main staff is only one person). We had a difficult time accomplishing our initial HW work. So in this part, we hope to be able to provide a profound lesson.
Before the actual production and debugging, full consideration and deduction must be carried out. The delivery of the things bought often cost two or three days (In China, we mainly shop online, so the delivery time needs to be considered), and the purchase of goods may be inconsistent with what we wanted. Plus, the negligence of some important details may also lead to the purchase of wrong things, which will waste a lot of valuable time.
If you want to know more or cooperate with us, welcome to contact us.