Difference between revisions of "Team:NPU-China/InterLab"

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                     <h2 style="text-align:center">Overview</h2>
 
                     <h2 style="text-align:center">Overview</h2>
 
                     <h4>The aim of the interlab this year was to explore--
 
                     <h4>The aim of the interlab this year was to explore--
−
                         <br> "How close can the numbers be when fluorescence is measured all around the world?"  
+
                         <br> "How close can the numbers be when fluorescence is measured all around the world?"
 
                         <br> Participating in the interlab was a valuable experience, and it allowed us to make use of technology
 
                         <br> Participating in the interlab was a valuable experience, and it allowed us to make use of technology
 
                         we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension
 
                         we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension
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                         <br>
 
                         <br>
 
                         <br> 1. OD600 reference point
 
                         <br> 1. OD600 reference point
−
                         <div align="center"><img src="https://static.igem.org/mediawiki/2017/c/c0/Interlab-1.1.png" class="img-responsive" width="40%" height="40%" ></div>
+
                         <div align="center">
 +
                            <img src="https://static.igem.org/mediawiki/2017/c/c0/Interlab-1.1.png" class="img-responsive" width="40%" height="40%">
 +
                        </div>
 
                         <br>
 
                         <br>
−
                        <br> 2. Fluorescein standard curve
 
−
                        <div align="center"><img src="https://static.igem.org/mediawiki/2017/9/91/Interlab-1.2.png" class="img-responsive" width="40%" height="40%" ></div>
 
−
                        <div align="center"><img src="https://static.igem.org/mediawiki/2017/a/a0/Interlab-2.1.png" class="img-responsive" width="40%" height="40%" ></div>
 
−
                       
 
−
                       
 
 
                         <br>
 
                         <br>
−
                         <br> 3. Raw Plate Reader Measurements</h4>
+
                         <br> 2. Fluorescein standard curve
−
                        <div align="center"><img src="https://static.igem.org/mediawiki/2017/2/2d/Interlab-2.2.png" class="img-responsive" width="40%" height="40%" ></div>
+
                        <div class="col-md-12">
−
                        <div align="center"><img src="https://static.igem.org/mediawiki/2017/5/58/Interlab-3.png" class="img-responsive" width="40%" height="40%" ></div>
+
                            <div class="col-md-6">
−
                     
+
                                <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab-1.2.png" class="img-responsive">
−
                          
+
                            </div>
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                    <br>
+
                            <div class="col-md-6">
−
                    <h2 style="text-align:center">Observation</h2>
+
                                <img src="https://static.igem.org/mediawiki/2017/a/a0/Interlab-2.1.png" class="img-responsive">
−
                    <h4>
+
                            </div>
−
                        1. It can be easily observed that Device1 and Device 4 didn’t grow after 6 hours. In fact, Device1 express high level of
+
                         </div>
−
                        GFP after being cultured all night long.
+
−
                        <br>
+
−
                        <br> 2.In Fluorescein standard curve, it’s not linear when the concentration is high. But the Fluorescein
+
−
                        in Raw Plate Reader Measurements doesn’t reach the the non-linear region so the standard curve is
+
−
                        believable.
+
−
                        <br>
+
−
                        <br> 3. It was found there was great variation in readings when using different settings on the plate
+
−
                        reader. Given that iGEM HQ has kept the protocol consistent, it is suggested that the desired plate
+
−
                        reader settings are also specified to ensure consistency throughout the results.
+
−
                        <br>
+
−
                        <br> 4. The dilution forum provided by iGEM foundation is useful. After dilution, almost every OD600
+
−
                        is around 0.04.
+
 
                     </h4>
 
                     </h4>
 +
 +
 +
                    <br>
 +
                    <br>
 +
                    <div style="padding-top:350px">
 +
                        <h4> 3. Raw Plate Reader Measurements</h4>
 +
                    </div>
 +
                    <div class="col-md-12">
 +
                        <div class="col-md-6">
 +
                            <img src="https://static.igem.org/mediawiki/2017/6/6d/Interlab_%281%29.jpg" class="img-responsive">
 +
                        </div>
 +
                        <div class="col-md-6">
 +
                            <img src="https://static.igem.org/mediawiki/2017/d/dd/Interlab_%282%29.jpg" class="img-responsive">
 +
                        </div>
 +
                    </div>
 +
                    <br>
 +
                    <br>
 +
                    <div class="col-md-12" style="padding-top:50px">
 +
                        <div class="col-md-6">
 +
                            <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab_%283%29.jpg" class="img-responsive">
 +
                        </div>
 +
                        <div class="col-md-6">
 +
                            <img src="https://static.igem.org/mediawiki/2017/f/f5/Interlab_%284%29.jpg" class="img-responsive">
 +
                        </div>
 +
                    </div>
 +
 +
 +
 +
                    <br>
 +
                    <br>
 +
                    <br>
 +
                    <br>
 +
                    <div style="padding-top:650px"></div>
 +
                        <h2 style="text-align:center" >Observation</h2>
 +
                        <h4>
 +
                            1. It can be easily observed that Device1 and Device 4 didn’t grow after 6 hours. In fact, Device1 express high level of
 +
                            GFP after being cultured all night long.
 +
                            <br>
 +
                            <br> 2.In Fluorescein standard curve, it’s not linear when the concentration is high. But the Fluorescein
 +
                            in Raw Plate Reader Measurements doesn’t reach the the non-linear region so the standard curve
 +
                            is believable.
 +
                            <br>
 +
                            <br> 3. It was found there was great variation in readings when using different settings on the plate
 +
                            reader. Given that iGEM HQ has kept the protocol consistent, it is suggested that the desired
 +
                            plate reader settings are also specified to ensure consistency throughout the results.
 +
                            <br>
 +
                            <br> 4. The dilution forum provided by iGEM foundation is useful. After dilution, almost every OD600
 +
                            is around 0.04.
 +
                        </h4>
  
 
                 </div>
 
                 </div>

Revision as of 03:14, 31 October 2017

Overview

The aim of the interlab this year was to explore--
"How close can the numbers be when fluorescence is measured all around the world?"
Participating in the interlab was a valuable experience, and it allowed us to make use of technology we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension to our lab work, and was a lot of fun!

1. OD600 reference point



2. Fluorescein standard curve



3. Raw Plate Reader Measurements







Observation

1. It can be easily observed that Device1 and Device 4 didn’t grow after 6 hours. In fact, Device1 express high level of GFP after being cultured all night long.

2.In Fluorescein standard curve, it’s not linear when the concentration is high. But the Fluorescein in Raw Plate Reader Measurements doesn’t reach the the non-linear region so the standard curve is believable.

3. It was found there was great variation in readings when using different settings on the plate reader. Given that iGEM HQ has kept the protocol consistent, it is suggested that the desired plate reader settings are also specified to ensure consistency throughout the results.

4. The dilution forum provided by iGEM foundation is useful. After dilution, almost every OD600 is around 0.04.