Difference between revisions of "Team:NPU-China/InterLab"

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{{NPU-China}}
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{{NPU-China/mmp}}
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<html lang="en">
  
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<head>
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    <!-- Bootstrap Core CSS -->
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    <link href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet">
  
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<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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    <link href="https://2017.igem.org/Template:NPU-China/css?action=raw&ctype=text/css" rel="stylesheet">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>InterLab</h1>
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    <link href="https://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet">
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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<br><br>
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
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                                <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a>
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                                <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a>
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                                <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a>
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                            <li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a>
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                            </li>
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                                <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a>
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                        <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a>
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                                <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a>
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                        <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a>
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                        <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a>
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                                <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a>
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    <!-- Page Content -->
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    <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;">
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        <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/a/a6/%E9%A2%98%E7%9B%AE%E5%B0%8F%E9%80%9A%E6%A0%8Finterlab.jpg">
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        <div class="container" style="padding-top:70px">
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            <div class="row">
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                <div class="col-md-12">
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                    <h2 style="text-align:center">Overview</h2>
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                    <h4>The aim of the interlab this year was to explore--
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                        <br> "How close can the numbers be when fluorescence is measured all around the world?"
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                        <br> Participating in the interlab was a valuable experience, and it allowed us to make use of technology
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                        we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension
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                        to our lab work, and was a lot of fun!
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                        <br>
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                        <br> 1. OD600 reference point
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                        <div align="center">
 +
                            <img src="https://static.igem.org/mediawiki/2017/c/c0/Interlab-1.1.png" class="img-responsive" width="40%" height="40%">
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                        </div>
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                        <br>
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                        <br>
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                        <br> 2. Fluorescein standard curve
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                        <div class="col-md-12">
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                            <div class="col-md-6">
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                                <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab-1.2.png" class="img-responsive">
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                            </div>
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                            <div class="col-md-6">
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                                <img src="https://static.igem.org/mediawiki/2017/a/a0/Interlab-2.1.png" class="img-responsive">
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                            </div>
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                        </div>
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                    </h4>
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                    <br>
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                    <br>
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                    <div style="padding-top:30px">
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                        <h4> 3. Raw Plate Reader Measurements</h4>
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                    </div>
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                    <div class="col-md-12">
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                        <div class="col-md-6">
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                            <img src="https://static.igem.org/mediawiki/2017/6/6d/Interlab_%281%29.jpg" class="img-responsive">
 +
                        </div>
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                        <div class="col-md-6">
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                            <img src="https://static.igem.org/mediawiki/2017/d/dd/Interlab_%282%29.jpg" class="img-responsive">
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                        </div>
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                    </div>
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                    <br>
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                    <br>
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                    <div class="col-md-12" style="padding-top:20px">
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                        <div class="col-md-6">
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                            <img src="https://static.igem.org/mediawiki/2017/9/91/Interlab_%283%29.jpg" class="img-responsive">
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                        </div>
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                        <div class="col-md-6">
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                            <img src="https://static.igem.org/mediawiki/2017/f/f5/Interlab_%284%29.jpg" class="img-responsive">
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                        </div>
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                    </div>
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                    <br>
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                    <br>
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                    <br>
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                    <br>
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                    <div style="padding-top:600px"></div>
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                        <h2 style="text-align:center" >Observation</h2>
 +
                        <h4>
 +
                            1. It can be easily observed that Device1 and Device 4 didn’t grow after 6 hours. In fact, Device1 express high level of
 +
                            GFP after being cultured all night long.
 +
                            <div align="center">
 +
                                <img src="https://static.igem.org/mediawiki/2017/f/ff/Interlab-gfp.jpg" class="img-responsive" width="40%" height="40%">
 +
                            </div>
 +
                           
 +
                            <br>
 +
                            <br> 2.In Fluorescein standard curve, it’s not linear when the concentration is high. But the Fluorescein
 +
                            in Raw Plate Reader Measurements doesn’t reach the the non-linear region so the standard curve
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                            is believable.
 +
                            <br>
 +
                            <br> 3. It was found there was great variation in readings when using different settings on the plate
 +
                            reader. Given that iGEM HQ has kept the protocol consistent, it is suggested that the desired
 +
                            plate reader settings are also specified to ensure consistency throughout the results.
 +
                            <br>
 +
                            <br> 4. The dilution forum provided by iGEM foundation is useful. After dilution, almost every OD600
 +
                            is around 0.04.
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                        </h4>
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                </div>
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        <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive">
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Latest revision as of 03:19, 31 October 2017

Overview

The aim of the interlab this year was to explore--
"How close can the numbers be when fluorescence is measured all around the world?"
Participating in the interlab was a valuable experience, and it allowed us to make use of technology we had at our disposal that otherwise wasn’t relevant to our project. It added an extra dimension to our lab work, and was a lot of fun!

1. OD600 reference point



2. Fluorescein standard curve



3. Raw Plate Reader Measurements







Observation

1. It can be easily observed that Device1 and Device 4 didn’t grow after 6 hours. In fact, Device1 express high level of GFP after being cultured all night long.


2.In Fluorescein standard curve, it’s not linear when the concentration is high. But the Fluorescein in Raw Plate Reader Measurements doesn’t reach the the non-linear region so the standard curve is believable.

3. It was found there was great variation in readings when using different settings on the plate reader. Given that iGEM HQ has kept the protocol consistent, it is suggested that the desired plate reader settings are also specified to ensure consistency throughout the results.

4. The dilution forum provided by iGEM foundation is useful. After dilution, almost every OD600 is around 0.04.