Difference between revisions of "Team:NPU-China/Model"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1> Modeling</h1>
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<p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
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<h3> Gold Medal Criterion #3</h3>
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To complete for the gold medal criterion #3, please describe your work on this page and fill out the description on your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>. To achieve this medal criterion, you must convince the judges that your team has gained insight into your project from modeling. You may not convince the judges if your model does not have an effect on your project design or implementation.  
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Please see the <a href="https://2017.igem.org/Judging/Medals"> 2017 Medals Page</a> for more information.
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Model prize</a>, please describe your work on this page  and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>. Please note you can compete for both the gold medal criterion #3 and the best model prize with this page.
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                    <li class="active"> <a href="https://2017.igem.org/Team:NPU-China">Home</a> </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li>
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                        </ul>
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                    </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li>
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                        </ul>
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                    </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> </li>
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                        </ul>
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                    </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> </li>
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a>
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                        <ul class="dropdown-menu">
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li>
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                        </ul>
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                    </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li>
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                    <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li>
  
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                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a>
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<h5> Inspiration </h5>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li>
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Here are a few examples from previous teams:
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li>
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</p>
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                        </ul>
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<ul>
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                    </li>
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<li><a href="https://2016.igem.org/Team:Manchester/Model">Manchester 2016</a></li>
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                </ul>
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<li><a href="https://2016.igem.org/Team:TU_Delft/Model">TU Delft 2016  </li>
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            </div>
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<li><a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">ETH Zurich 2014</a></li>
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        </div>
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<li><a href="https://2014.igem.org/Team:Waterloo/Math_Book">Waterloo 2014</a></li>
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                    <h2 class="page-header" align="center" >Abstract</h2>
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                    <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
 +
                        polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 +
                        annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
 +
                        US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which
 +
                        is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution,
 +
                        high energy consumption and it is unsustainable.<br> This year, we aim to use a green and environmentally
 +
                        friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional
 +
                        chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.
 +
                    </h4>
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                    <h2 class="page-header" align="center">We construct cell factory based on 4 levels, which are—</h2>
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                    <br>
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            </div>
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                    </a>
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                    <h3 align="center">
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                        <a href="portfolio-item.html">Core Part</a>
 +
                    </h3>
 +
                    <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type
 +
                        's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic
 +
                        effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design)
 +
                        platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography)
 +
                        and HTS(High throughput screening).
 +
                    </h4>
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                    </a>
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                    <h3 align="center">
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                        <a href="portfolio-item.html">System</a>
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                    </h3>
 +
                    <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient
 +
                        to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms,
 +
                        we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually.
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                    </h4>
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                    </a>
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                        <a href="portfolio-item.html">Pathway</a>
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                    </h3>
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                    <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different
 +
                        optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol
 +
                        metabolism of the two bacteria, so that it could produce the target product acrylic acid using the
 +
                        intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed
 +
                        and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway
 +
                        and reduce the loss of bypass carbon flux.</h4>
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                    </a>
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                    <h3 align="center">
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                        <a href="portfolio-item.html" >Production</a>
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                    </h3>
 +
                    <h4>All of the previous processes are to build the engineering strains which have a high production of acrylic
 +
                        acid that we need. In the subsequent fermentation, we also need to determine the best parameters
 +
                        of the engineering strain.<br> Therefore, we selected the carbon source, Buffer, temperature, pH
 +
                        and other conditions to optimize the cell production process.</h4>
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Revision as of 13:30, 27 October 2017

Abstract

Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution, high energy consumption and it is unsustainable.
This year, we aim to use a green and environmentally friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.

We construct cell factory based on 4 levels, which are—


Core Part

We use ceaS2 enzyme as the core part, but acrylic acid is a byproduct of ceaS2 enzyme, the wild type 's catalytic effect is very weak, whose production is only 1mg/L. So we hope to improve the catalytic effect of ceaS2 enzyme.
We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for better-worked ceaS2 mutants by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).

System

Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, we have them both tested, which were E. coli MG1655 and S. cerevisiae BY4741 individually.

Pathway

We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes for them.We introduced the ceaS2 enzyme exogenously on the basis of the glycerol metabolism of the two bacteria, so that it could produce the target product acrylic acid using the intermediates G3P and DHAP.Besides having finished the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduce the loss of bypass carbon flux.

Production

All of the previous processes are to build the engineering strains which have a high production of acrylic acid that we need. In the subsequent fermentation, we also need to determine the best parameters of the engineering strain.
Therefore, we selected the carbon source, Buffer, temperature, pH and other conditions to optimize the cell production process.