Shuimoliuyun (Talk | contribs) |
|||
Line 466: | Line 466: | ||
<br> | <br> | ||
We first selected the mutations within the 5Å distance of active site, altogether 33 kinds, and then used point mutation to conduct molecular cloning operation. Next step was to synthesize the acrylic acid using the whole cell catalysis and determined the acrylic acid yield by HPLC. The results are as follows: <br> | We first selected the mutations within the 5Å distance of active site, altogether 33 kinds, and then used point mutation to conduct molecular cloning operation. Next step was to synthesize the acrylic acid using the whole cell catalysis and determined the acrylic acid yield by HPLC. The results are as follows: <br> | ||
− | <center> <img src="https://static.igem.org/mediawiki/2017/ | + | <center> <img src="https://static.igem.org/mediawiki/2017/e/e4/NPU-26.png" class="img-responsive"></center> |
In these total 33 mutations of mutational sites, there are 11 mutations' acrylic acid yield higher than that of the wild type, which indicates a higher activity. The highest mutational site F438M presents a yield 11 times the wild type. Therefore, it is valid and tangible for us to implement AEMD to design the mutational sites! | In these total 33 mutations of mutational sites, there are 11 mutations' acrylic acid yield higher than that of the wild type, which indicates a higher activity. The highest mutational site F438M presents a yield 11 times the wild type. Therefore, it is valid and tangible for us to implement AEMD to design the mutational sites! | ||
</h4> | </h4> |
Revision as of 20:47, 1 November 2017