Difference between revisions of "Team:Peking/Contribution"

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            <!-- Title -->
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            <a href="https://2017.igem.org/Team:Peking"><img
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            <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking"
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              style="color: #000000; font-size: x-large"><strong>Peking iGEM </strong> 2017</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking">Home</a>
 +
                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Project">Project</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Model">Modelling</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Software">Software</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Hardware">Hardware</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Lab">Lab</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/HP" style="color: #000; font-weight: 500;">Practices</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Parts">Parts</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Team">Team</a>
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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
 
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<h1>Contribution</h1>
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<h3>Bronze Medal Criterion #4</h3>
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        }
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
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                <h1>Contribution <br><br> </h1><br>
 +
 
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 +
 
 +
 
 +
                <strong>This year, Peking iGEM parcitipated in the Fourth InterLaboratory Measurement Study in synthetic biology, along with teams around the world. And we also improved the characterization of an existing BioBrick Part <a
 +
                        href="http://parts.igem.org/Part:BBa_K1132010"
 +
                        target="blank"
 +
                        style="color: #226CE4"><strong>BBa_K1132010</strong></a>
 +
                    and submitted the improved part <a
 +
                            href="http://parts.igem.org/Part:BBa_K2243000"
 +
                            target="blank"
 +
                            style="color: #226CE4"><strong>BBa_K2243000</strong></a>.</strong>
 +
 
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<br><br>
 
<br><br>
−
<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
+
                    <li><strong>InterLab</strong>
 +
                        <br><br>
 +
                        Reliable and repeatable measurement is a key component of synthetic biology. However, there have been few opportunities to repeat the same measurements in different labs. In order to quantify the degree of variability exhibited by engineered genetic constructs across different laboratories, the measurement committee invited all iGEM teams to participate in the Interlab study, which provides researchers with a detailed protocol and data analysis form, with the aim to produce common, comparable units for measuring GFP on different plate readers.
 +
                        We introduced the required eight plasmids into E. coli K-12 DH5-alpha, measured the GFP expression levels using a plate reader, submitted the results on time and display them on <a
 +
                                href="https://2017.igem.org/Team:Peking/InterLab"
 +
                                target="blank"
 +
                                style="color: #226CE4"><strong>InterLab</strong></a> Page.
 +
                    </li>
 +
<br><br>
 +
                    <li><strong>Part improvement</strong>
 +
                        <br><br>
 +
                        TP901-1 integrase is widely used according to literature research, but the quantitative information of this part(<a
 +
                            href="http://parts.igem.org/Part:BBa_K1132010"
 +
                            target="blank"
 +
                            style="color: #226CE4"><strong>BBa_K1132010</strong></a>) is very limited. This year we hope to use the integrase to control the direction of other biological parts, thus influencing the function of the cells.
 +
                        So we have characterized it using different promoters and RBSes. Through the tuning procedure, we can express TP901-1 integrase well and invert DNA efficiently.(See <a
 +
                                href="http://parts.igem.org/Part:BBa_K2243000"
 +
                                target="blank"
 +
                                style="color: #226CE4"><strong>BBa_K2243000</strong></a> for details)
 +
                    </li>
  
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                <h1 class="mdl-mega-footer__heading">Acknowledgement</h1>
 +
                <ul class="mdl-mega-footer__link-list" style="margin-left: 0px;>
 +
                    <li><a href=" http:
 +
                //dean.pku.edu.cn/pkudean/"> </li>
 +
                <li><a href="http://dean.pku.edu.cn/pkudean/"><img
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 +
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 +
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 +
                </li>
 +
                <li><a href="http://cqb.pku.edu.cn/en/"> <img
 +
                        src="https://static.igem.org/mediawiki/2017/9/9a/Peking_footer_logo_cqb.png" height="48px"/> </a></li>
 +
 
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                <h1 class="mdl-mega-footer__heading">Connection</h1>
 +
                <ul class="mdl-mega-footer__link-list" style="margin-left: 0px;>
 +
                    <li><a href=" https:
 +
                //www.facebook.com/pekingigem"></a></li>
 +
                <li><a href="https://www.facebook.com/pekingigem">Facebook</a></li>
 +
                <li><a href="https://www.youtube.com/channel/UCefEYVCVqKJLmoJrSxivgZQ">Youtube</a></li>
 +
                <li><a href="https://twitter.com/iGEM_Peking">Twitter</a></li>
 +
                <li><a href="https://github.com/PekingiGEM2017">Github</a></li>
 +
                </ul>
 +
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 +
                    <li><a href=" http:
 +
                //www.synbiowiki.com"> </a></li>
 +
                <li><a href="http://www.synbiowiki.com">SynBioWiki.com</a></li>
 +
                <li><a href="mailto:PekingIGEM@gmail.com">Mail</a></li>
 +
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                <h2 style="color: white; "><strong>Peking iGEM</strong> 2017</h2>
 +
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 +
                    <li style="color: white; line-height: 1.88em;">Peking University,<br> No.5 Yiheyuan Road Haidian
 +
                        District, Beijing, P.R.China<br>100871
 +
                    </li>
 +
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Latest revision as of 19:37, 1 November 2017

Peking iGEM 2017

Contribution


This year, Peking iGEM parcitipated in the Fourth InterLaboratory Measurement Study in synthetic biology, along with teams around the world. And we also improved the characterization of an existing BioBrick Part BBa_K1132010 and submitted the improved part BBa_K2243000.


  1. InterLab

    Reliable and repeatable measurement is a key component of synthetic biology. However, there have been few opportunities to repeat the same measurements in different labs. In order to quantify the degree of variability exhibited by engineered genetic constructs across different laboratories, the measurement committee invited all iGEM teams to participate in the Interlab study, which provides researchers with a detailed protocol and data analysis form, with the aim to produce common, comparable units for measuring GFP on different plate readers. We introduced the required eight plasmids into E. coli K-12 DH5-alpha, measured the GFP expression levels using a plate reader, submitted the results on time and display them on InterLab Page.


  2. Part improvement

    TP901-1 integrase is widely used according to literature research, but the quantitative information of this part(BBa_K1132010) is very limited. This year we hope to use the integrase to control the direction of other biological parts, thus influencing the function of the cells. So we have characterized it using different promoters and RBSes. Through the tuning procedure, we can express TP901-1 integrase well and invert DNA efficiently.(See BBa_K2243000 for details)