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margin-bottom: 0px; | margin-bottom: 0px; | ||
} | } | ||
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<link rel="stylesheet" type="text/css" | <link rel="stylesheet" type="text/css" | ||
href="https://2017.igem.org/Template:Peking/mdl/icon?action=raw&ctype=text/css"> | href="https://2017.igem.org/Template:Peking/mdl/icon?action=raw&ctype=text/css"> | ||
− | |||
</head> | </head> | ||
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<!-- Always shows a header, even in smaller screens. --> | <!-- Always shows a header, even in smaller screens. --> | ||
<div class="mdl-layout mdl-js-layout mdl-layout--fixed-header"> | <div class="mdl-layout mdl-js-layout mdl-layout--fixed-header"> | ||
− | <header class="mdl-layout__header"> | + | <header class="mdl-layout__header" style="position:fixed; margin-top: -7px; !important;"> |
<div class="mdl-layout__header-row"> | <div class="mdl-layout__header-row"> | ||
<!-- Title --> | <!-- Title --> | ||
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</style> | </style> | ||
<a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking">Home</a> | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking">Home</a> | ||
− | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Project">Project</a> | + | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Project#Introduction">Project</a> |
− | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Model">Modelling</a> | + | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Model#Overview">Modelling</a> |
<a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Software">Software</a> | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Software">Software</a> | ||
<a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Hardware">Hardware</a> | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Hardware">Hardware</a> | ||
− | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Lab" style="color: #000; font-weight: 500;">Lab</a> | + | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Lab" |
+ | style="color: #000; font-weight: 500;">Lab</a> | ||
<a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/HP">Practices</a> | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/HP">Practices</a> | ||
− | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Parts">Parts</a> | + | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Parts#Overview">Parts</a> |
<a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Team">Team</a> | <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Team">Team</a> | ||
</nav> | </nav> | ||
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</div> | </div> | ||
− | <main class="docs-layout-content mdl-layout__content mdl-color-text--grey-600" style="width: 100%; padding-bottom: 0px; background-color: #FAFAFA;"> | + | <main class="docs-layout-content mdl-layout__content mdl-color-text--grey-600" |
+ | style="width: 100%; padding-bottom: 0px; background-color: #FAFAFA;"> | ||
<!-- Wide card with share menu button --> | <!-- Wide card with share menu button --> | ||
<style> | <style> | ||
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width: auto; | width: auto; | ||
} | } | ||
+ | |||
.demo-card-wide > .mdl-card__title { | .demo-card-wide > .mdl-card__title { | ||
color: #fff; | color: #fff; | ||
height: 360px; | height: 360px; | ||
} | } | ||
+ | |||
.demo-card-wide > .mdl-card__menu { | .demo-card-wide > .mdl-card__menu { | ||
color: #fff; | color: #fff; | ||
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<div class="demo-card-wide mdl-card mdl-shadow--2dp"> | <div class="demo-card-wide mdl-card mdl-shadow--2dp"> | ||
− | <div class="mdl-card__title" style="background: url('https://static.igem.org/mediawiki/2017/ | + | <div class="mdl-card__title" |
+ | style="background: url('https://static.igem.org/mediawiki/2017/0/0c/Peking_Safety2.jpeg') center / cover; padding-left: 50px;"> | ||
<div class="mdl-card__title-text"> | <div class="mdl-card__title-text"> | ||
− | <h1 style="font-size: xx-large; color: white"><strong>Safety</strong></h1> | + | <h1 style="font-size: xx-large; color: white; text-shadow:2px 2px 8px #070707;" ><strong>Safety</strong></h1> |
</div> | </div> | ||
</div> | </div> | ||
− | + | <div class="mdl-card__supporting-text" | |
− | <div class="mdl-card__supporting-text" style="line-height: 2em;text-align: justify; color: #3A3A3A; padding-left: 50px; padding-top: 50px; padding-bottom:50px"> | + | style="line-height: 2em;text-align: justify; color: #3A3A3A; padding-left: 50px; padding-top: 50px; padding-bottom:50px"> |
<section class="docs-toc docs-text-styling"> | <section class="docs-toc docs-text-styling"> | ||
<nav class="section-content"> | <nav class="section-content"> | ||
<ul style="margin-left: 0px;"> | <ul style="margin-left: 0px;"> | ||
− | <li><a href="# | + | <li><a href="#p2">Safe Project Design</a> |
− | + | ||
− | + | ||
</li> | </li> | ||
− | <li><a href=" | + | <li><a href="#p3">Safe Lab Work</a> |
</li> | </li> | ||
− | <li><a href=" | + | <li><a href="#p4">Safe Shipment</a> |
</li> | </li> | ||
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</section> | </section> | ||
<br> | <br> | ||
− | + | This year, the Peking iGEM Team is trying to develop a framework of sequential logic circuits in cells. | |
− | + | Here we are going to share our experience in safety control.<br><br> | |
− | + | <h2 id="p2">Safe Project Design</h2> | |
− | + | <strong>Choosing a non-pathogenic chassis<br></strong> | |
− | + | In our project, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning | |
− | + | and protein expression.<br> | |
− | + | <strong>Choosing parts that will be environmental friendly<br></strong> | |
− | + | Harmful parts are not allowed in our project. The parts in our project are mostly commonly used recombinases | |
− | + | and expression control units (such as terminators and promoters widely used in research), so that none of | |
− | + | our parts would raise any safety issues according to current professional knowledge.<br> | |
− | + | <br><h2 id="p3">Safe Lab Work</h2> | |
− | + | According to the requirements of iGEM policy, in our daily bench work we never performed any dangerous | |
− | + | experiments or faced any unusual safety issues. Our bench work followed some basic regulations as | |
− | + | follows:<br> | |
− | + | <ol start="1"> | |
− | + | <script type="text/javascript"> | |
− | + | (function () { | |
− | + | ||
− | + | var doc_ols = document.getElementsByTagName("ol"); | |
− | + | ||
− | + | for (i = 0; i < doc_ols.length; i++) { | |
− | + | ||
− | + | var ol_start = doc_ols[i].getAttribute("start") - 1; | |
− | + | doc_ols[i].setAttribute("style", "counter-reset:ol " + ol_start + ";"); | |
− | + | ||
− | + | } | |
− | + | ||
− | + | })(); | |
− | + | </script> | |
− | + | <li>Duplicating a key to the laboratory without permission is strictly prohibited.</li> | |
− | + | <li>Participants need to understand the experiment completely.</li> | |
− | + | <li>Wear rubber gloves during all experimenters.</li> | |
− | + | <li>Steps with any probability of contamination should be performed in a biosafety cabinet.</li> | |
− | + | <li>Fire, electric heaters, and microwave ovens should never be left unattended.</li> | |
− | + | <li>All liquid and solid waste containing living organisms must be autoclaved.</li> | |
− | + | <li>Sanitize the lab using UV-light every week.</li> | |
− | + | <li>The last person to leave the lab should make sure that water, electricity, gas, and air conditioners | |
− | + | have been turned off, and doors and windows have been locked before leaving. | |
− | + | </li> | |
− | + | </ol> | |
− | + | <br> | |
− | + | <h2 id="p4">Safe Shipment</h2> | |
− | + | As mentioned above, our DNA parts are absolutely safe because they encode safe proteins such as Bxb1 gp35 | |
− | + | and their combinations with promoters/terminators. The DNA parts are safely contained within PCR tubes as | |
− | + | the Parts registry requires.<br> | |
− | <a class="mdl-button mdl-js-button mdl-button--raised mdl-js-ripple-effect" href = "https://2017.igem.org/Team:Peking/Lab" style="background-color: #CB2C32; color: white;"> | + | |
+ | |||
+ | <br><br><br><br> | ||
+ | <a class="mdl-button mdl-js-button mdl-button--raised mdl-js-ripple-effect" | ||
+ | href="https://2017.igem.org/Team:Peking/Lab" style="background-color: #CB2C32; color: white;"> | ||
Back < Lab | Back < Lab | ||
</a> | </a> | ||
<a id="demo-menu-top-left" | <a id="demo-menu-top-left" | ||
− | class="mdl-button mdl-button--accent mdl-js-button mdl-button--raised mdl-js-ripple-effect" style="background-color:orangered; color: white;"> | + | class="mdl-button mdl-button--accent mdl-js-button mdl-button--raised mdl-js-ripple-effect" |
+ | style="background-color:orangered; color: white;"> | ||
Goto > Lab/... | Goto > Lab/... | ||
</a> | </a> | ||
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<ul class="mdl-menu mdl-menu--top-left mdl-js-menu mdl-js-ripple-effect" | <ul class="mdl-menu mdl-menu--top-left mdl-js-menu mdl-js-ripple-effect" | ||
data-mdl-for="demo-menu-top-left" style="margin-left: 0px"> | data-mdl-for="demo-menu-top-left" style="margin-left: 0px"> | ||
− | <a class="mdl-menu__item" href="https://2017.igem.org/Team:Peking/Notebook" style="color: #000;">Notebook</a> | + | <a class="mdl-menu__item" href="https://2017.igem.org/Team:Peking/Notebook" |
+ | style="color: #000;">Notebook</a> | ||
<a class="mdl-menu__item" href="https://2017.igem.org/Team:Peking/Protocols" style="color: #000;">Protocols</a> | <a class="mdl-menu__item" href="https://2017.igem.org/Team:Peking/Protocols" style="color: #000;">Protocols</a> | ||
− | <a class="mdl-menu__item" href="https://2017.igem.org/Team:Peking/InterLab" style="color: #000;">InterLab</a> | + | <a class="mdl-menu__item" href="https://2017.igem.org/Team:Peking/InterLab" |
+ | style="color: #000;">InterLab</a> | ||
</ul> | </ul> | ||
− | |||
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<h1 class="mdl-mega-footer__heading">Acknowledgement</h1> | <h1 class="mdl-mega-footer__heading">Acknowledgement</h1> | ||
<ul class="mdl-mega-footer__link-list" style="margin-left: 0px;> | <ul class="mdl-mega-footer__link-list" style="margin-left: 0px;> | ||
− | <li><a href="http://dean.pku.edu.cn/pkudean/"> </li> | + | <li><a href=" http: |
− | <li><a href="http://dean.pku.edu.cn/pkudean/"><img src="https://static.igem.org/mediawiki/2017/9/99/Peking_footer_logo_admin.png" height = "50px"/></a></li> | + | //dean.pku.edu.cn/pkudean/"> </li> |
− | <li><a href="http://www.bio.pku.edu.cn/en/"> <img src="https://static.igem.org/mediawiki/2017/a/ab/Peking_footer_logo_slslogo.png" height = "50px"/> </a></li> | + | <li><a href="http://dean.pku.edu.cn/pkudean/"><img |
− | <li><a href="http://cqb.pku.edu.cn/en/"> <img src="https://static.igem.org/mediawiki/2017/9/9a/Peking_footer_logo_cqb.png" height = "48px"/> </a></li> | + | src="https://static.igem.org/mediawiki/2017/9/99/Peking_footer_logo_admin.png" height="50px"/></a> |
+ | </li> | ||
+ | <li><a href="http://www.bio.pku.edu.cn/en/"> <img | ||
+ | src="https://static.igem.org/mediawiki/2017/a/ab/Peking_footer_logo_slslogo.png" height="50px"/> </a> | ||
+ | </li> | ||
+ | <li><a href="http://cqb.pku.edu.cn/en/"> <img | ||
+ | src="https://static.igem.org/mediawiki/2017/9/9a/Peking_footer_logo_cqb.png" height="48px"/> </a></li> | ||
</ul> | </ul> | ||
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<input class="mdl-mega-footer__heading-checkbox" type="checkbox" checked> | <input class="mdl-mega-footer__heading-checkbox" type="checkbox" checked> | ||
<h1 class="mdl-mega-footer__heading">Connection</h1> | <h1 class="mdl-mega-footer__heading">Connection</h1> | ||
− | <ul class="mdl-mega-footer__link-list" | + | <ul class="mdl-mega-footer__link-list" style="margin-left: 0px;> |
− | <li><a href="https://www.facebook.com/pekingigem"></a></li> | + | <li><a href=" https: |
+ | //www.facebook.com/pekingigem"></a></li> | ||
<li><a href="https://www.facebook.com/pekingigem">Facebook</a></li> | <li><a href="https://www.facebook.com/pekingigem">Facebook</a></li> | ||
<li><a href="https://www.youtube.com/channel/UCefEYVCVqKJLmoJrSxivgZQ">Youtube</a></li> | <li><a href="https://www.youtube.com/channel/UCefEYVCVqKJLmoJrSxivgZQ">Youtube</a></li> | ||
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<h1 class="mdl-mega-footer__heading">Contact</h1> | <h1 class="mdl-mega-footer__heading">Contact</h1> | ||
<ul class="mdl-mega-footer__link-list" style="margin-left: 0px;> | <ul class="mdl-mega-footer__link-list" style="margin-left: 0px;> | ||
− | <li><a href="http://www.synbiowiki.com"> </a></li> | + | <li><a href=" http: |
+ | //www.synbiowiki.com"> </a></li> | ||
<li><a href="http://www.synbiowiki.com">SynBioWiki.com</a></li> | <li><a href="http://www.synbiowiki.com">SynBioWiki.com</a></li> | ||
<li><a href="mailto:PekingIGEM@gmail.com">Mail</a></li> | <li><a href="mailto:PekingIGEM@gmail.com">Mail</a></li> | ||
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<div class="mdl-mega-footer__drop-down-section"> | <div class="mdl-mega-footer__drop-down-section"> | ||
<input class="mdl-mega-footer__heading-checkbox" type="checkbox" checked> | <input class="mdl-mega-footer__heading-checkbox" type="checkbox" checked> | ||
− | <h2 style="color: white; "> <strong>Peking iGEM</strong> 2017</h2> | + | <h2 style="color: white; "><strong>Peking iGEM</strong> 2017</h2> |
<ul class="mdl-mega-footer__link-list" style="margin-left: 0px;"> | <ul class="mdl-mega-footer__link-list" style="margin-left: 0px;"> | ||
− | <li style="color: white; line-height: 1.88em;">Peking University,<br> No.5 Yiheyuan Road Haidian District, Beijing, P.R.China<br>100871</li> | + | <li style="color: white; line-height: 1.88em;">Peking University,<br> No.5 Yiheyuan Road Haidian |
+ | District, Beijing, P.R.China<br>100871 | ||
+ | </li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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</main> | </main> | ||
− | |||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 13:42, 1 November 2017
Safety
This year, the Peking iGEM Team is trying to develop a framework of sequential logic circuits in cells. Here we are going to share our experience in safety control.
Safe Project Design
Choosing a non-pathogenic chassisIn our project, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning and protein expression.
Choosing parts that will be environmental friendly
Harmful parts are not allowed in our project. The parts in our project are mostly commonly used recombinases and expression control units (such as terminators and promoters widely used in research), so that none of our parts would raise any safety issues according to current professional knowledge.
Safe Lab Work
According to the requirements of iGEM policy, in our daily bench work we never performed any dangerous experiments or faced any unusual safety issues. Our bench work followed some basic regulations as follows:- Duplicating a key to the laboratory without permission is strictly prohibited.
- Participants need to understand the experiment completely.
- Wear rubber gloves during all experimenters.
- Steps with any probability of contamination should be performed in a biosafety cabinet.
- Fire, electric heaters, and microwave ovens should never be left unattended.
- All liquid and solid waste containing living organisms must be autoclaved.
- Sanitize the lab using UV-light every week.
- The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.
Safe Shipment
As mentioned above, our DNA parts are absolutely safe because they encode safe proteins such as Bxb1 gp35 and their combinations with promoters/terminators. The DNA parts are safely contained within PCR tubes as the Parts registry requires.Back < Lab Goto > Lab/...