Difference between revisions of "Team:Peking/Safety"

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<h1> Safety </h1>
 
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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        /***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
  
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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            background-color: #FAFAFA;
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<li>Choosing parts that will not harm humans / animals / plants</li>
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        !important;
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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        }
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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            <!-- Title -->
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            <a href="https://2017.igem.org/Team:Peking"><img
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                    src="https://static.igem.org/mediawiki/2017/c/cf/Peking_TeamLogoClassical.png" height="35"></a>
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            <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking"
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              style="color: #000000; font-size: x-large"><strong>Peking iGEM </strong> 2017</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking">Home</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Project#Introduction">Project</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Model#Overview">Modelling</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Software">Software</a>
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                  style="color: #000; font-weight: 500;">Lab</a>
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                <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Peking/Parts#Overview">Parts</a>
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            </nav>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
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        <div class="mdl-card__title"
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                <h1 style="font-size: xx-large; color: white; text-shadow:2px 2px 8px #070707;" ><strong>Safety</strong></h1>
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                        <li><a href="#p2">Safe Project Design</a>
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                        </li>
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                        <li><a href="#p3">Safe Lab Work</a>
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                        </li>
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                        <li><a href="#p4">Safe Shipment</a>
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                        </li>
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                    </ul>
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            </section>
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            <br>
 +
            This year, the Peking iGEM Team is trying to develop a framework of sequential logic circuits in cells.
 +
            Here we are going to share our experience in safety control.<br><br>
 +
            <h2 id="p2">Safe Project Design</h2>
 +
            <strong>Choosing a non-pathogenic chassis<br></strong>
 +
            In our project, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning
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            and protein expression.<br>
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            <strong>Choosing parts that will be environmental friendly<br></strong>
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            Harmful parts are not allowed in our project. The parts in our project are mostly commonly used recombinases
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            and expression control units (such as terminators and promoters widely used in research), so that none of
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            our parts would raise any safety issues according to current professional knowledge.<br>
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            <br><h2 id="p3">Safe Lab Work</h2>
 +
            According to the requirements of iGEM policy, in our daily bench work we never performed any dangerous
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            experiments or faced any unusual safety issues. Our bench work followed some basic regulations as
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            follows:<br>
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                <li>Duplicating a key to the laboratory without permission is strictly prohibited.</li>
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                <li>Participants need to understand the experiment completely.</li>
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                <li>Wear rubber gloves during all experimenters.</li>
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                <li>Steps with any probability of contamination should be performed in a biosafety cabinet.</li>
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                <li>Fire, electric heaters, and microwave ovens should never be left unattended.</li>
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                <li>All liquid and solid waste containing living organisms must be autoclaved.</li>
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                <li>Sanitize the lab using UV-light every week.</li>
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                <li>The last person to leave the lab should make sure that water, electricity, gas, and air conditioners
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                    have been turned off, and doors and windows have been locked before leaving.
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                </li>
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            </ol>
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            <br>
 +
            <h2 id="p4">Safe Shipment</h2>
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            As mentioned above, our DNA parts are absolutely safe because they encode safe proteins such as Bxb1 gp35
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            and their combinations with promoters/terminators. The DNA parts are safely contained within PCR tubes as
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            the Parts registry requires.<br>
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            <br><br><br><br>
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            <a class="mdl-button mdl-js-button mdl-button--raised mdl-js-ripple-effect"
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                Back < Lab
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                Goto > Lab/...
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                  style="color: #000;">Notebook</a>
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                <h1 class="mdl-mega-footer__heading">Acknowledgement</h1>
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                    <li><a href=" http:
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                //dean.pku.edu.cn/pkudean/"> </li>
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                    <li><a href=" https:
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                //www.facebook.com/pekingigem"></a></li>
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                <li><a href="https://www.facebook.com/pekingigem">Facebook</a></li>
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                <li><a href="https://www.youtube.com/channel/UCefEYVCVqKJLmoJrSxivgZQ">Youtube</a></li>
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                <li><a href="https://twitter.com/iGEM_Peking">Twitter</a></li>
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                <li><a href="https://github.com/PekingiGEM2017">Github</a></li>
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                    <li><a href=" http:
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                //www.synbiowiki.com"> </a></li>
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                <li><a href="http://www.synbiowiki.com">SynBioWiki.com</a></li>
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                <li><a href="mailto:PekingIGEM@gmail.com">Mail</a></li>
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                <h2 style="color: white; "><strong>Peking iGEM</strong> 2017</h2>
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                    <li style="color: white; line-height: 1.88em;">Peking University,<br> No.5 Yiheyuan Road Haidian
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                        District, Beijing, P.R.China<br>100871
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Latest revision as of 13:42, 1 November 2017

Peking iGEM 2017

Safety


This year, the Peking iGEM Team is trying to develop a framework of sequential logic circuits in cells. Here we are going to share our experience in safety control.

Safe Project Design

Choosing a non-pathogenic chassis
 In our project, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning and protein expression.
Choosing parts that will be environmental friendly
 Harmful parts are not allowed in our project. The parts in our project are mostly commonly used recombinases and expression control units (such as terminators and promoters widely used in research), so that none of our parts would raise any safety issues according to current professional knowledge.

Safe Lab Work

According to the requirements of iGEM policy, in our daily bench work we never performed any dangerous experiments or faced any unusual safety issues. Our bench work followed some basic regulations as follows:
  1. Duplicating a key to the laboratory without permission is strictly prohibited.
  2. Participants need to understand the experiment completely.
  3. Wear rubber gloves during all experimenters.
  4. Steps with any probability of contamination should be performed in a biosafety cabinet.
  5. Fire, electric heaters, and microwave ovens should never be left unattended.
  6. All liquid and solid waste containing living organisms must be autoclaved.
  7. Sanitize the lab using UV-light every week.
  8. The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.

Safe Shipment

As mentioned above, our DNA parts are absolutely safe because they encode safe proteins such as Bxb1 gp35 and their combinations with promoters/terminators. The DNA parts are safely contained within PCR tubes as the Parts registry requires.




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