Overview

This year, the Peking iGEM Team is trying to develop a framework of se-quential logic circuits in cells. Here we are going to share our experience in safety control.

Safe Project Design

Choosing a non-pathogenic chassis

In our project, non-pathogenic strains of E. coli were the only chassis used to perform molecular cloning and protein expression.

Choosing parts that will be environmental friendly

Harmful parts are not allowed in our project. The parts in our project are mostly commonly used recombinases and expression control units (such as terminators and promoters widely used in research), so that none of our parts would raise any safety issues according to current professional knowledge.

Safe Lab Work

According to the requirements of iGEM policy, in our daily bench work we never performed any dangerous experiments or faced any unusual safety issues. Our bench work followed some basic regulations as follows:

1. Duplicating a key to the laboratory without permission is strictly prohibit-ed.
2. Participants need to understand the experiment completely.
3. Wear rubber gloves during all experimenters.
4. Steps with any probability of contamination should be performed in a bio-safety cabinet.
5. Fire, electric heaters, and microwave ovens should never be left unat-tended.
6. All liquid and solid waste containing living organisms must be auto-claved.
7. Sanitize the lab using UV-light every week.
8. The last person to leave the lab should make sure that water, electricity, gas, and air conditioners have been turned off, and doors and windows have been locked before leaving.

Safe Shipment

As mentioned above, our DNA parts are absolutely safe because they en-code safe proteins such as Bxb1 gp35 and their combinations with pro-moters/terminators. The DNA parts are safely contained within PCR tubes as the Parts registry requires.

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