Team:Pittsburgh/Demonstrate

This set of charts demonstrate the motility of transformed bacterial cells exposed to a 500nm light source which monomerizes the dronpa proteins within the bacterial cells containing the dronpa protein (WT CheY-D and Mut CheY-D). These cells should exhibit tumbling behavior as the CheY protein should be capable of binding to the FliM proteins within the flagellar motor. In the CheY KO cells and DH5-alpha cells exposed to the 500nm light source, since they do not contain the dronpa protein, they will exhibit the “random walk” phase of bacterial chemotaxis and run and tumble at about the same frequency. This chart elucidates that our constructs that are designed to tumble under 500nm light have approximately the same motility as “normal, untransformed” bacterial cells used as controls. Therefore, we can interpret this data by stating that it is inconclusive, from this data set, whether or not dronpa monomerization has controlled bacterial chemotaxis in any significant way as compared to the negative controls in this experimental design.