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<iframe src="https://drive.google.com/file/d/0B8DtFUN9dEIgZDRIWHgxdVVmWnM/preview" width="480" height="360"></iframe> | <iframe src="https://drive.google.com/file/d/0B8DtFUN9dEIgZDRIWHgxdVVmWnM/preview" width="480" height="360"></iframe> | ||
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+ | CheY Wild-type Dronpa Fusion | ||
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+ | <iframe src="https://drive.google.com/file/d/0B8DtFUN9dEIgTXJfNDlDYlFyRm8/preview" width="640" height="480"></iframe> | ||
+ | <iframe src="https://drive.google.com/file/d/0B8DtFUN9dEIgcmRvVktBbXR5Y2s/preview" width="640" height="480"></iframe> | ||
+ | <iframe src="https://drive.google.com/file/d/0B8DtFUN9dEIgZ2dsRVV3czZJRU0/preview" width="640" height="480"></iframe> | ||
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+ | CheY Mutant | ||
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+ | <iframe src="https://drive.google.com/file/d/0B8DtFUN9dEIgTXJfNDlDYlFyRm8/preview" width="640" height="480"></iframe> | ||
+ | <iframe src="https://drive.google.com/file/d/0B8DtFUN9dEIgcmRvVktBbXR5Y2s/preview" width="640" height="480"></iframe> | ||
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Revision as of 23:07, 8 December 2017
Fluorescence Results
Dronpa is a photoswitchable fluorescent protein that loses fluorescence under 500-nm light and restores fluorescence under 400-nm light. In our fluorescence assays, we wanted to assess if our constructs are expressing Dronpa.
Plate Reader Fluorescence Assay
Microscopy Videos
In order: 1. Pre-illumination 2. After 2 minutes 500nm light 3. After 3 seconds 400nm light
CheY Knockout
CheY Wild-type
CheY Wild-type Dronpa Fusion
CheY Mutant