Difference between revisions of "Team:Pittsburgh/Results"

Line 10: Line 10:
  
 
<h3>Plate Reader Fluorescence Assay</h3>
 
<h3>Plate Reader Fluorescence Assay</h3>
<p?
+
<p>
 
<img src="https://static.igem.org/mediawiki/2017/f/f7/T--Pittsburgh--microchart.jpeg" height="400" width="600">
 
<img src="https://static.igem.org/mediawiki/2017/f/f7/T--Pittsburgh--microchart.jpeg" height="400" width="600">
 
</p>
 
</p>

Revision as of 23:05, 8 December 2017

Fluorescence Results

Dronpa is a photoswitchable fluorescent protein that loses fluorescence under 500-nm light and restores fluorescence under 400-nm light. In our fluorescence assays, we wanted to assess if our constructs are expressing Dronpa.

Plate Reader Fluorescence Assay


Microscopy Videos

In order: 1. Pre-illumination 2. After 2 minutes 500nm light 3. After 3 seconds 400nm light

CheY Knockout



CheY Wild-type