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Revision as of 23:08, 8 December 2017

Fluorescence Results

Dronpa is a photoswitchable fluorescent protein that loses fluorescence under 500-nm light and restores fluorescence under 400-nm light. In our fluorescence assays, we wanted to assess if our constructs are expressing Dronpa.

Plate Reader Fluorescence Assay


Microscopy Videos

In order: 1. Pre-illumination 2. After 2 minutes 500nm light 3. After 3 seconds 400nm light

CheY Knockout



CheY Wild-type



CheY Wild-type Dronpa Fusion



CheY Mutant