Team:Pittsburgh/Results

Fluorescence Results

Dronpa is a photoswitchable fluorescent protein that loses fluorescence under 500-nm light and restores fluorescence under 400-nm light. In our fluorescence assays, we wanted to assess if our constructs are expressing Dronpa.

Plate Reader Fluorescence Assay

Microscopy Videos

In order: 1. Pre-illumination 2. After 2 minutes 500nm light 3. After 3 seconds 400nm light

CheY Knockout



CheY Wild-type