Difference between revisions of "Team:SJTU-BioX-Shanghai/Basic Part-cn"
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Revision as of 09:22, 10 December 2017
基本元件总体介绍
今年我们队伍将选择另一个修改过的STAR——STAR3作为今年的基本模块(BBa_K2285020)。 我们今年的项目是建立在2016年帝国理工学校STAR系统的基础的（为了区分我们和他们的工作，我们把他们的STAR系统称为STAR1），然后我们决定建立另一套STAR系统（我们称之为STAR3）来达到多因子检测的目的。简单来说就是两套STAR系统可以控制两个基因回路。更多关于STAR优势和工作原理的信息可以在 这里查阅。
Figure 1. The STAR construct. In the absence of antisense, sense target RNA will form a stem-loop structure, functioning as a terminator and stop the following transcription. When antisense RNA, a mRNA fragment
which is the antisense of part of the target RNA, is transcribed, the terminator structure will be disrupted and switch on the inhibited transcription.
为了之后描述的方便，我们在这里引入一些名词用于指代各个原件:
在帝国理工的项目里，基因上游的茎环结构被称为STAR-Target，我们简略称为Target，其互补序列我们称之为Antisense。当我们提到Target1和Antisense1的时候，我们指的是帝国理工构建的那套系统。当我们提到Target3和Antisense3的时候，我们说的是我们今年项目构建的系统。
请浏览我们的 设计页面来获得更多关于STAR3系统设计的信息
另外可以浏览 Target 3 BBa_K2285020 原件页面来获取更多关于STAR系统表征的信息 (Both STAR 1 and STAR 3) system.
我们提交的基本模块
| Part Number | Type | Description | Length (bp) |
| BBa_K2285010 | RNA | Antisense3+t500 | 104 |
| BBa_K2285020 | Regulatory | J23119+Target3 | 128 |
Reference
- Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs. Nat Chem Biol 11:214–220.
- Meyer, S., Chappell, J., Sankar, S., Chew, R., and Lucks, J. B. (2016) Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies Biotechnol. Bioeng. 113, 216.
