Difference between revisions of "Team:TP-CC San Diego"

 
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{{:Team:TP-CC_San_Diego/Templates/NavBar}}
 
{{:Team:TP-CC_San_Diego/Templates/NavBar}}
 
{{:Team:TP-CC San Diego/Templates/Font}}
 
{{:Team:TP-CC San Diego/Templates/Font}}
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{{:Team:TP-CC_San_Diego/Templates/AnimationCSS}}
  
 
<html>
 
<html>
 
<head>
 
<head>
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<title>Home</title>
  
 
<link href="https://cdn.rawgit.com/michalsnik/aos/2.1.1/dist/aos.css" rel="stylesheet">
 
<link href="https://cdn.rawgit.com/michalsnik/aos/2.1.1/dist/aos.css" rel="stylesheet">
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.main-title {
 
.main-title {
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   padding:40px 80px 40px 80px;
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   padding: 40px 80px 40px 80px;
 
   font-size: 50px;
 
   font-size: 50px;
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   margin-top: 7%;
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   padding-top: 6%;
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  color: #333333;
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}
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.inner-title
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{
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}
 
}
  
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to{opacity:1}}
 
to{opacity:1}}
  
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.icon-wrapper
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{
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  display: inline-block;
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  margin: 1%;
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}
  
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                #outer{
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.icon
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height: 500px;
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{
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width: 100%;
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  width: 100%;
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background-image: url("https://static.igem.org/mediawiki/2017/4/41/TP-CC_San_Diego_Safety_Picture_2.jpeg");
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  height: 100%;
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background-size: cover;
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  margin: 0 auto auto auto;
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margin: 7% 0px 40px 0px;
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}
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                        data-aos: fade-right;
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}
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#outerRight{
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.icon img
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height: 500px;
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{
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width: 100%;
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  position: block;
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background-image: url("https://static.igem.org/mediawiki/2017/4/41/TP-CC_San_Diego_Safety_Picture_2.jpeg");
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  width: 100%;
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background-size: cover;
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  height: 100%;
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margin: 0px 0px 20px 0px;
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}
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                        data-aos: fade-left;
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}
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#inner{
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.icon-wrapper h3
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width: 1100px;
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{
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height: 500px;
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  position: relative;
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                        overflow: hidden;
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  margin: auto;
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margin: 0 auto 0 auto;
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  top: -15%;
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}
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  text-align: center;
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}
  
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#finalinner{
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.imageDiv
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width: 300px;
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{
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height: 700px;
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    display: inline-block;
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float: left;
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padding: 0px 75px 0 35px;
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}
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background-color: white;
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}
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#finalinnerRight{
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width: 300px;
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height: 700px;
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float: right;
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padding: 0px 35px 0 75px;
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text-align: right;
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background-color: white;
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}
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#inner a{
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.info
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color: #243249;
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font-weight: 700;
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font-size: 12px;
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font-family: Montserrat;
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border: 2px solid #243249;
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padding: 5px 10px 5px 10px;
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#inner a:hover{
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color: white;
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font-family: Montserrat;
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background-color: #243249;
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border: 2px solid #243249;
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padding: 5px 10px 5px 10px;
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text-decoration: none;
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                        font-family: 'Montserrat', sans-serif;
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}
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.meetteam
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{
 
{
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   margin: 2% auto auto auto;
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  font-family: 'Poppins', sans-serif;
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  color: black;
 
   text-align: center;
 
   text-align: center;
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  font-family: 'Montserrat', sans-serif;
 
 
}
 
}
  
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.meetteam a{
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.banner
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color: #243249;
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{
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font-weight: 700;
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font-size: 15px;
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}
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font-family: Montserrat;
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border: 2px solid #243249;
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padding: 7px 12px 7px 12px;
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}
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.meetteam a:hover{
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.text-wrapper1
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color: white;
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{
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font-weight: 700;
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    background: none;
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font-size: 15px;
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    margin: 80px 0px 0px 2%;
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font-family: Montserrat;
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    padding: 2% 2% 2% 2%;
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background-color: #243249;
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    width: 80%;
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border: 2px solid #243249;
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    height: 100%;
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padding: 7px 12px 7px 12px;
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    margin: auto;
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text-decoration: none;
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}
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}
 
</style>
 
</style>
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</head>
 
</head>
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<body>
 
<body>
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<h2 class = "main-title fade-in">ecDNA</h2>
 
  
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<div id="outer" data-aos="fade-left" class="aos-init aos-animate">
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<center>
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<div id="inner">
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<div class="banner">
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<div id="finalinner">
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<img style="width: 100%;margin: auto auto .5% auto;" src="https://static.igem.org/mediawiki/2017/archive/1/11/20171029225547%21T--TP-CC_San_Diego--banner.png">
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<h2 style="margin-bottom: 0px; margin-top: 20px;" class = "inner-title" >PROJECT OVERVIEW</h2>
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<div id="borderBot"></div><br><br>
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<p style="margin-bottom: 15px;">
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Cancer is mostly caused by somatic mutations acquired throughout an individual’s lifetime.  Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.
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</p>
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<a class="findout" style = "text-align: center" href="https://2017.igem.org/Team:TP-CC_San_Diego/Description">FIND OUT MORE</a>
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</div>
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</div>
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</div>
 
</div>
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</center>
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<!--<center>
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<h2 class = "main-title fade-in" style="margin: 5% 0 5% 0;">ecDNA</h2>
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-->
  
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<div id="outerRight" data-aos="fade-right" aos-duration="1000">
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<div id="inner">
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<div class="text-wrapper1">
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<div id="finalinnerRight">
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  <h3 class="day" style="margin-top: 2%;">Abstract</h3>
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<h2 style="margin-bottom: 0px; margin-top: 20px;" class = "inner-title">NOTEBOOK</h2>
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<p style="font-size: 22px; margin-top: 20px;">
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<div id="borderBot" style="float: right;"></div><br><br>
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Cancer, a genetic disease resulting in uncontrollable cell growth, is mostly caused by somatic mutations acquired throughout an individual’s lifetime. Because it induces the increased expression of growth related genes, oncogene amplification is one of the driving forces of cancer cell replication. Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Like the DNA on chromosomes, ecDNAs are double stranded. A key difference, however, is the circular shape of ecDNAs; they are able to randomly distribute because they do not have centromeres, which increases heterogeneity in daughter cells. This can cause the cancerous tumors to develop faster resistance to current treatments. To target the ecDNA, we used CRISPR technology to create double strand breaks specifically in the ecDNA. Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.
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<p style="margin-bottom: 15px;">Over several months in the lab, we've gone from concept to completion in creating an E.coli strain that synthesizes and secretes chitinase. Have an inside look at the processes and procedures that led to our success. </p>
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<a  href="https://2017.igem.org/Team:TP-CC_San_Diego/LabBook">FIND OUT MORE</a>
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</p>
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        </div>
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        </div>
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        </div>
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<div class="meetteam">
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<h2 class="subtitle" style="width: 300px;">
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THE TEAM MEMBERS OF TP_CC-SANDIEGO iGEM THAT MADE THIS PROJECT POSSIBLE
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</h2>
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<a href="https://2017.igem.org/Team:TP-CC_San_Diego/Team">MEET THE TEAM</a>
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</div>
 
</div>
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<center>
 +
<div style="margin-top: 2%;">
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Description">
 +
        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e4/20171023064510%21T--TP-CC_San_Diego--brain.png" width = "250"></div>
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        <div class="info">
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        <h3 class = "page-title">Project Description</h3>
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        </div></a>
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</div>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Results">
 +
        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e1/20171023064527%21T--TP-CC_San_Diego--beaker.png" width = "250"></div>
 +
        <div class="info">
 +
      <h3 class = "page-title">Results</h3>
 +
        </div></a>
 +
</div>
 +
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Model">
 +
        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/6/6a/20171023064653%21T--TP-CC_San_Diego--graph.png" width = "250"></div>
 +
        <div class="info">
 +
      <h3 class = "page-title">Modeling</h3>
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        </div></a>
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</div>
 +
</div>
 +
</center>
 +
   
 +
<center>
 +
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/LabBook">
 +
        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/d/d7/20171023064353%21T--TP-CC_San_Diego--book.png" width = "250"></div>
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        <div class="info">
 +
      <h3 class = "page-title">Lab Notebook</h3>
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        </div></a>
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</div>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/InterLab">
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        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/2/2d/20171023064557%21T--TP-CC_San_Diego--cell.png" width = "250"></div>
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        <div class="info">
 +
      <h3 class = "page-title">Interlab Study</h3>
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        </div></a>
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</div>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Protocols">
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        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/0/03/20171023064720%21T--TP-CC_San_Diego--magnifying.png" width = "250"></div>
 +
        <div class="info">
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      <h3 class = "page-title">Protocols</h3>
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        </div></a>
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</div>
 +
</center>
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 +
<center>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/MedalCriteria">
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        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/4/4a/20171023064737%21T--TP-CC_San_Diego--medal.png" width = "250"></div>
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        <div class="info">
 +
      <h3 class = "page-title">Medal Criteria</h3>
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        </div></a>
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</div>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/HumanPractices">
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        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e7/20171023064751%21T--TP-CC_San_Diego--podium.png" width = "250"></div>
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        <div class="info">
 +
      <h3 class = "page-title">Human Practices</h3>
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        </div></a>
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</div>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Team">
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        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e7/20171023064541%21T--TP-CC_San_Diego--ecdnagroup.png" width = "250"></div>
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        <div class="info">
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      <h3 class = "page-title">Team</h3>
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        </div></a>
 
</div>
 
</div>
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</center>
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 00:04, 2 November 2017

Home

Abstract

Cancer, a genetic disease resulting in uncontrollable cell growth, is mostly caused by somatic mutations acquired throughout an individual’s lifetime. Because it induces the increased expression of growth related genes, oncogene amplification is one of the driving forces of cancer cell replication. Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Like the DNA on chromosomes, ecDNAs are double stranded. A key difference, however, is the circular shape of ecDNAs; they are able to randomly distribute because they do not have centromeres, which increases heterogeneity in daughter cells. This can cause the cancerous tumors to develop faster resistance to current treatments. To target the ecDNA, we used CRISPR technology to create double strand breaks specifically in the ecDNA. Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.

Lab Notebook

Interlab Study

Protocols

Medal Criteria

Human Practices

Team