Difference between revisions of "Team:TP-CC San Diego"

 
(136 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{:Team:TP-CC_San_Diego/Templates/NavBar}}
 
{{:Team:TP-CC_San_Diego/Templates/NavBar}}
 
{{:Team:TP-CC San Diego/Templates/Font}}
 
{{:Team:TP-CC San Diego/Templates/Font}}
 +
{{:Team:TP-CC_San_Diego/Templates/AnimationCSS}}
 +
 
<html>
 
<html>
 
<head>
 
<head>
Line 12: Line 14:
  
 
<style>
 
<style>
−
</head>
 
  
 
.main-title {
 
.main-title {
 
   padding: 40px 80px 40px 80px;
 
   padding: 40px 80px 40px 80px;
 
   font-size: 50px;
 
   font-size: 50px;
−
   margin-top: 6%;
+
   padding-top: 6%;
 
}
 
}
  
Line 26: Line 27:
 
from{opacity:0}  
 
from{opacity:0}  
 
to{opacity:1}}
 
to{opacity:1}}
−
 
−
.container
 
−
{
 
−
  width: 93%;
 
−
  height: 100%;
 
−
  background-color: none;
 
−
  margin-left: auto;
 
−
  margin-right: auto;
 
−
}
 
  
 
.icon-wrapper
 
.icon-wrapper
 
{
 
{
 
   display: inline-block;
 
   display: inline-block;
−
   width: 27.3%;
+
   margin: 1%;
−
  height: 450px;
+
−
  backgroud-color: none;
+
−
  padding: 3%;
+
−
  float: left;
+
−
  text-align: center;
+
 
}
 
}
  
 
.icon
 
.icon
 
{
 
{
−
   width:100%;
+
   width: 100%;
−
   height: 80%;
+
   height: 100%;
 
   margin: 0 auto auto auto;
 
   margin: 0 auto auto auto;
 
}
 
}
Line 56: Line 43:
 
.icon img
 
.icon img
 
{
 
{
−
   width: 400px;
+
  position: block;
−
   height: 386px;
+
   width: 100%;
 +
   height: 100%;
 
}
 
}
  
Line 71: Line 59:
 
{
 
{
 
     display: inline-block;
 
     display: inline-block;
−
    padding: 1%;
+
−
 
+
−
}
+
−
 
+
−
.textDiv
+
−
{
+
−
    text-align: center;
+
−
    top: -20px;
+
 
}
 
}
  
−
.page-title
+
.info
 
{
 
{
−
   margin:30px auto auto auto;
+
   margin: 2% auto auto auto;
 
   font-size: 20px;
 
   font-size: 20px;
 
   font-family: 'Poppins', sans-serif;
 
   font-family: 'Poppins', sans-serif;
 
   color: black;
 
   color: black;
 
   text-align: center;
 
   text-align: center;
−
  border-width: 3px;
 
−
  border-color: black;
 
−
  border-style: solid;
 
−
  padding: 3%;
 
 
}
 
}
  
 +
.banner
 +
{
 +
  padding-top: -1%;
 +
}
  
 +
.text-wrapper1
 +
{
 +
    background: none;
 +
    margin: 80px 0px 0px 2%;
 +
    padding: 2% 2% 2% 2%;
 +
    width: 80%;
 +
    height: 100%;
 +
    margin: auto;
 +
 +
}
 
</style>
 
</style>
−
 
+
</head>
 
<body>
 
<body>
 +
 +
<center>
 +
<div class="banner">
 +
<img style="width: 100%;margin: auto auto .5% auto;" src="https://static.igem.org/mediawiki/2017/archive/1/11/20171029225547%21T--TP-CC_San_Diego--banner.png">
 +
</div>
 +
</center>
 +
<!--<center>
 
<h2 class = "main-title fade-in" style="margin: 5% 0 5% 0;">ecDNA</h2>
 
<h2 class = "main-title fade-in" style="margin: 5% 0 5% 0;">ecDNA</h2>
 +
-->
 +
 +
 +
<div class="text-wrapper1">
 +
  <h3 class="day" style="margin-top: 2%;">Abstract</h3>
 +
<p style="font-size: 22px; margin-top: 20px;">
 +
Cancer, a genetic disease resulting in uncontrollable cell growth, is mostly caused by somatic mutations acquired throughout an individual’s lifetime. Because it induces the increased expression of growth related genes, oncogene amplification is one of the driving forces of cancer cell replication. Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Like the DNA on chromosomes, ecDNAs are double stranded. A key difference, however, is the circular shape of ecDNAs; they are able to randomly distribute because they do not have centromeres, which increases heterogeneity in daughter cells. This can cause the cancerous tumors to develop faster resistance to current treatments. To target the ecDNA, we used CRISPR technology to create double strand breaks specifically in the ecDNA. Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.
 +
 +
</p>
 +
</div>
  
 
<center>
 
<center>
−
<div class="imageDiv">
+
<div style="margin-top: 2%;">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/e/e4/20171023064510%21T--TP-CC_San_Diego--brain.png" width = "400">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Description">
−
         <div class="textDiv">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e4/20171023064510%21T--TP-CC_San_Diego--brain.png" width = "250"></div>
 +
         <div class="info">
 
         <h3 class = "page-title">Project Description</h3>
 
         <h3 class = "page-title">Project Description</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
  
−
<div class="imageDiv">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Results">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/e/e1/20171023064527%21T--TP-CC_San_Diego--beaker.png" width = "400">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e1/20171023064527%21T--TP-CC_San_Diego--beaker.png" width = "250"></div>
−
         <div class="textDiv">
+
         <div class="info">
 
       <h3 class = "page-title">Results</h3>
 
       <h3 class = "page-title">Results</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
  
−
<div class="imageDiv">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Model">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/2/2d/20171023064557%21T--TP-CC_San_Diego--cell.png" width = "400">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/6/6a/20171023064653%21T--TP-CC_San_Diego--graph.png" width = "250"></div>
−
         <div class="textDiv">
+
         <div class="info">
−
       <h3 class = "page-title">Proof</h3>
+
       <h3 class = "page-title">Modeling</h3>
−
         </div>
+
         </div></a>
 +
</div>
 
</div>
 
</div>
 
</center>
 
</center>
−
 
+
   
 
<center>
 
<center>
−
<div class="imageDiv">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/LabBook">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/6/6a/20171023064653%21T--TP-CC_San_Diego--graph.png" width = "400">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/d/d7/20171023064353%21T--TP-CC_San_Diego--book.png" width = "250"></div>
−
         <div class="textDiv">
+
         <div class="info">
−
       <h3 class = "page-title">Modeling</h3>
+
       <h3 class = "page-title">Lab Notebook</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
  
−
<div class="imageDiv">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/InterLab">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/d/d7/20171023064353%21T--TP-CC_San_Diego--book.png" width = "400">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/2/2d/20171023064557%21T--TP-CC_San_Diego--cell.png" width = "250"></div>
−
         <div class="textDiv">
+
         <div class="info">
−
       <h3 class = "page-title">Lab Notebook</h3>
+
       <h3 class = "page-title">Interlab Study</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
−
 
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Protocols">
−
<div class="imageDiv">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/0/03/20171023064720%21T--TP-CC_San_Diego--magnifying.png" width = "250"></div>
−
         <img src="https://static.igem.org/mediawiki/2017/archive/0/03/20171023064720%21T--TP-CC_San_Diego--magnifying.png" width = "400">
+
         <div class="info">
−
         <div class="textDiv">
+
 
       <h3 class = "page-title">Protocols</h3>
 
       <h3 class = "page-title">Protocols</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
 
</center>
 
</center>
−
 
+
   
 
<center>
 
<center>
−
<div class="imageDiv">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/MedalCriteria">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/4/4a/20171023064737%21T--TP-CC_San_Diego--medal.png" width = "400">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/4/4a/20171023064737%21T--TP-CC_San_Diego--medal.png" width = "250"></div>
−
         <div class="textDiv">
+
         <div class="info">
 
       <h3 class = "page-title">Medal Criteria</h3>
 
       <h3 class = "page-title">Medal Criteria</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
  
−
<div class="imageDiv">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/HumanPractices">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/e/e7/20171023064751%21T--TP-CC_San_Diego--podium.png" width = "400">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e7/20171023064751%21T--TP-CC_San_Diego--podium.png" width = "250"></div>
−
         <div class="textDiv">
+
         <div class="info">
 
       <h3 class = "page-title">Human Practices</h3>
 
       <h3 class = "page-title">Human Practices</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
  
−
<div class="imageDiv">
+
<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Team">
−
         <img src="https://static.igem.org/mediawiki/2017/archive/e/e7/20171023064541%21T--TP-CC_San_Diego--ecdnagroup.png" width = "400">
+
         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e7/20171023064541%21T--TP-CC_San_Diego--ecdnagroup.png" width = "250"></div>
−
         <div class="textDiv">
+
         <div class="info">
 
       <h3 class = "page-title">Team</h3>
 
       <h3 class = "page-title">Team</h3>
−
         </div>
+
         </div></a>
 
</div>
 
</div>
 
</center>
 
</center>
−
 
−
 
−
 
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 00:04, 2 November 2017

Home

Abstract

Cancer, a genetic disease resulting in uncontrollable cell growth, is mostly caused by somatic mutations acquired throughout an individual’s lifetime. Because it induces the increased expression of growth related genes, oncogene amplification is one of the driving forces of cancer cell replication. Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Like the DNA on chromosomes, ecDNAs are double stranded. A key difference, however, is the circular shape of ecDNAs; they are able to randomly distribute because they do not have centromeres, which increases heterogeneity in daughter cells. This can cause the cancerous tumors to develop faster resistance to current treatments. To target the ecDNA, we used CRISPR technology to create double strand breaks specifically in the ecDNA. Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.

Lab Notebook

Interlab Study

Protocols

Medal Criteria

Human Practices

Team