Difference between revisions of "Team:TP-CC San Diego"

 
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{{:Team:TP-CC San Diego/Templates/Font}}
 
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{{:Team:TP-CC_San_Diego/Templates/AnimationCSS}}
 
{{:Team:TP-CC_San_Diego/Templates/AnimationCSS}}
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{
 
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{
 
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.info
 
{
 
{
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}
 
}
  
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.banner
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{
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.text-wrapper1
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{
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<body>
 
<body>
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<center>
 
<center>
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<h2 class = "main-title fade-in" style="margin: 5% 0 5% 0;">ecDNA</h2>
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<div class="banner">
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<img style="width: 100%;margin: auto auto .5% auto;" src="https://static.igem.org/mediawiki/2017/archive/1/11/20171029225547%21T--TP-CC_San_Diego--banner.png">
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<!--<center>
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<h2 class = "main-title fade-in" style="margin: 5% 0 5% 0;">ecDNA</h2>
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<div class="imageDiv ih-item circle effect13 top_to_bottom"><a href="#">
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         <img src="https://static.igem.org/mediawiki/2017/archive/e/e4/20171023064510%21T--TP-CC_San_Diego--brain.png" width = "350">
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<div class="text-wrapper1">
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  <h3 class="day" style="margin-top: 2%;">Abstract</h3>
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<p style="font-size: 22px; margin-top: 20px;">
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Cancer, a genetic disease resulting in uncontrollable cell growth, is mostly caused by somatic mutations acquired throughout an individual’s lifetime. Because it induces the increased expression of growth related genes, oncogene amplification is one of the driving forces of cancer cell replication. Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Like the DNA on chromosomes, ecDNAs are double stranded. A key difference, however, is the circular shape of ecDNAs; they are able to randomly distribute because they do not have centromeres, which increases heterogeneity in daughter cells. This can cause the cancerous tumors to develop faster resistance to current treatments. To target the ecDNA, we used CRISPR technology to create double strand breaks specifically in the ecDNA. Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.
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</p>
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</div>
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<center>
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<div style="margin-top: 2%;">
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Description">
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         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e4/20171023064510%21T--TP-CC_San_Diego--brain.png" width = "250"></div>
 
         <div class="info">
 
         <div class="info">
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        <div class="info-back">
 
 
         <h3 class = "page-title">Project Description</h3>
 
         <h3 class = "page-title">Project Description</h3>
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         </div></a>
 
         </div></a>
 
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</div>
  
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Results">
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         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e1/20171023064527%21T--TP-CC_San_Diego--beaker.png" width = "250"></div>
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         <div class="textDiv">
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         <div class="info">
 
       <h3 class = "page-title">Results</h3>
 
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         </div></a>
 
</div>
 
</div>
  
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Model">
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         <img src="https://static.igem.org/mediawiki/2017/archive/2/2d/20171023064557%21T--TP-CC_San_Diego--cell.png" width = "350">
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         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/6/6a/20171023064653%21T--TP-CC_San_Diego--graph.png" width = "250"></div>
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         <div class="info">
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       <h3 class = "page-title">Proof</h3>
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       <h3 class = "page-title">Modeling</h3>
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<center>
 
<center>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/LabBook">
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        <img src="https://static.igem.org/mediawiki/2017/archive/6/6a/20171023064653%21T--TP-CC_San_Diego--graph.png" width = "350">
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         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/d/d7/20171023064353%21T--TP-CC_San_Diego--book.png" width = "250"></div>
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         <div class="info">
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<div class="imageDiv">
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        <img src="https://static.igem.org/mediawiki/2017/archive/d/d7/20171023064353%21T--TP-CC_San_Diego--book.png" width = "350">
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         <div class="textDiv">
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       <h3 class = "page-title">Lab Notebook</h3>
 
       <h3 class = "page-title">Lab Notebook</h3>
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         </div></a>
 
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/InterLab">
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         <img src="https://static.igem.org/mediawiki/2017/archive/0/03/20171023064720%21T--TP-CC_San_Diego--magnifying.png" width = "350">
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         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/2/2d/20171023064557%21T--TP-CC_San_Diego--cell.png" width = "250"></div>
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        <div class="info">
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      <h3 class = "page-title">Interlab Study</h3>
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        </div></a>
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</div>
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/Protocols">
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        <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/0/03/20171023064720%21T--TP-CC_San_Diego--magnifying.png" width = "250"></div>
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         <div class="info">
 
       <h3 class = "page-title">Protocols</h3>
 
       <h3 class = "page-title">Protocols</h3>
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         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/4/4a/20171023064737%21T--TP-CC_San_Diego--medal.png" width = "250"></div>
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       <h3 class = "page-title">Medal Criteria</h3>
 
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         </div></a>
 
</div>
 
</div>
  
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<div class="icon-wrapper ih-item circle effect10 top_to_bottom"><a href="https://2017.igem.org/Team:TP-CC_San_Diego/HumanPractices">
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         <div class="info">
 
       <h3 class = "page-title">Human Practices</h3>
 
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         <div class="icon-wrapper img"><img src="https://static.igem.org/mediawiki/2017/archive/e/e7/20171023064541%21T--TP-CC_San_Diego--ecdnagroup.png" width = "250"></div>
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       <h3 class = "page-title">Team</h3>
 
       <h3 class = "page-title">Team</h3>
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Latest revision as of 00:04, 2 November 2017

Home

Abstract

Cancer, a genetic disease resulting in uncontrollable cell growth, is mostly caused by somatic mutations acquired throughout an individual’s lifetime. Because it induces the increased expression of growth related genes, oncogene amplification is one of the driving forces of cancer cell replication. Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Like the DNA on chromosomes, ecDNAs are double stranded. A key difference, however, is the circular shape of ecDNAs; they are able to randomly distribute because they do not have centromeres, which increases heterogeneity in daughter cells. This can cause the cancerous tumors to develop faster resistance to current treatments. To target the ecDNA, we used CRISPR technology to create double strand breaks specifically in the ecDNA. Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.

Lab Notebook

Interlab Study

Protocols

Medal Criteria

Human Practices

Team