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Modeling

CRISPR/Cas9 System

The CRISPR/Cas9 system can be used for gene editing purposed including inserting genes, deleting genes, and creating breaks in the DNA.

In our first three test groups, we will only be using one guide RNA for each cell culture. The CRISPR/Cas9 will create a double strand break at the target sequence, leaving the ecDNA in two pieces. breaks in the DNA.

In our 4th test group, we will be using 3 guide RNAs for each cell culture. This will create double strand breaks at 3 target sequences, leaving the ecDNA in 6 pieces. We would like to test this because the more breaks we create in the ecDNA, the harder it is for the ecDNA to ligate back together.