Difference between revisions of "Team:UCL"

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                             <p style="font-size: 18px; font-weight: 200" class="intro-homepage">UCL iGEM 2017 presents</p>
 
                             <p style="font-size: 18px; font-weight: 200" class="intro-homepage">UCL iGEM 2017 presents</p>
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<p class="intro-homepage">LIT(Light Induced Technologies)</p>
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<p class="intro-homepage">LIT (Light Induced Technologies)</p>
 
                             <p class="text-content-new">We developed applications for biological light switches. The mission was to standardise optogenetic tools for wider use in synthetic biology and to show how we can apply them in tissue engineering, building architectural structures and producing bacterial light bulbs.</p>
 
                             <p class="text-content-new">We developed applications for biological light switches. The mission was to standardise optogenetic tools for wider use in synthetic biology and to show how we can apply them in tissue engineering, building architectural structures and producing bacterial light bulbs.</p>
 
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<p class="pop-text">We combined a photosensitive protein with co-culturing E. Coli and cyanobacteria to design a bioluminescent light bulb. During day time, the photosensitive protein suppresses the bioluminescent ability of engineered E. Coli, while cyanobacteria photosynthesise and produce the necessary glucose for their neighbours. Through our conceptual prototype, we achieve both powerful illumination and sustainability in our light-bulb.</p>
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<p class="pop-text">We developed and prototyped a bacterial light-bulb - one that uses light-repressible bioluminescence and co-culturing to create an efficient and sustainable solution for public illumination. <i>E. coli</i> is engineered to regulate its bioluminescence in response to sunlight levels using a photosensitive protein and a blue-light repressible promoter. These cells will be co-cultured with photosynthetic cyanobacteria, <i>Synechococcus elongatus</i>, engineered to secrete sucrose through heterologous sucrose transporters.</p>
 
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<p class="pop-text">We used a <span style="color: red">photosensitive protein</span> and a <span style="color: green">photocaged non-natural amino acid</span> to make 2 light-induced gene activation systems for a 3D printing technology that only uses bacteria as raw material. Guided by light, cells form <span style="color: #24b4ff">3D structures</span> through adhering to each other and then produce the <span style="color: black">PHB biopolymer</span>, secreting it and layering the material in the already formed structure.</p>
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<p class="pop-text">We developed transcriptional and post-translational light switches for the induction of cellular aggregation. Transcriptional control of cell aggregation is achieved through the synthesis of our cell aggregation proteins under the control of our blue light inducible promoter and a blue light sensitive transcriptional inducer protein. Post-translational control is achieved by photocaging one of our proteins involved in cell aggregation using an unnatural amino acid, rendering it inactive unless exposed to UV light. Guided by light, cells will be directed to form 3D structures by adhering to each other and simultaneously producing and binding a desired polymer (PHA/silicates).</p>
 
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Revision as of 14:11, 26 October 2017

UCL LIT

UCL iGEM 2017 presents

LIT (Light Induced Technologies)

We developed applications for biological light switches. The mission was to standardise optogenetic tools for wider use in synthetic biology and to show how we can apply them in tissue engineering, building architectural structures and producing bacterial light bulbs.

We used biological light switches to design an organ printing system

We developed and prototyped a bacterial light-bulb - one that uses light-repressible bioluminescence and co-culturing to create an efficient and sustainable solution for public illumination. E. coli is engineered to regulate its bioluminescence in response to sunlight levels using a photosensitive protein and a blue-light repressible promoter. These cells will be co-cultured with photosynthetic cyanobacteria, Synechococcus elongatus, engineered to secrete sucrose through heterologous sucrose transporters.

We developed transcriptional and post-translational light switches for the induction of cellular aggregation. Transcriptional control of cell aggregation is achieved through the synthesis of our cell aggregation proteins under the control of our blue light inducible promoter and a blue light sensitive transcriptional inducer protein. Post-translational control is achieved by photocaging one of our proteins involved in cell aggregation using an unnatural amino acid, rendering it inactive unless exposed to UV light. Guided by light, cells will be directed to form 3D structures by adhering to each other and simultaneously producing and binding a desired polymer (PHA/silicates).