Team:UCL/Parts

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Basic parts

Part	Purpose
BBa_K2332002: PBlind promoter	Promoter activated by EL222 (BBa_K2332004) upon blue-light exposure (465nm)
BBa_K2332004: EL222	Light-sensitive DNA binding protein
BBa_K2332010: Intimin'-SpyTag	Cell surface display of SpyTag
BBa_K2332012: Intimin'-SpyCatcher	Cell surface display of SpyCatcher
BBa_K2332017: GFP-SpyTag	For visual localization of SpyTag
BBa_K2332030: Intimin'-GFP-SpyTag	Cell surface display and visual localization of of SpyTag
BBa_K2332031: GFP-SpyCatcher	For visual localization of SpyCatcher
BBa_K2332032: Photocaged GFP-SpyCatcher	Photocaging and visual localization of SpyCatcher
BBa_K2332015: Photocaged Intimin'-SpyCatcher	Photocaging SpyCatcher for UV-induced cell aggregation
BBa_K2332019: PBLrep promoter	Blue light repressible promoter
BBa_K2332020: LuxCDABE	Bacterial luminescence
BBa_K2332311: PhoCl	Mammalian photocleavable linker
BBa_K2332312: E-cadherin (Preproprotein)	Cell-cell adhesion
BBa_K2332314: E-cadherin-PhoCl Fusion	Light-activated cell-cell adhesion
BBa_K2332315: Plasma membrane delivery system	Coding gene for expression of target protein on the cytosolic leaflet of the plasma membrane and its photo-activated release into the cytoplasm (test construct for BBa_K2332317)
BBa_K2332316: dCas9-p65	Programmable transcription factor for reporter/target gene activation (test construct for BBa_K2332317)
BBa_K2332317: Photoactive dCas9-p65 (siLACE system)	Light-activated programmable transcription factor