Difference between revisions of "Team:UCSC/Safety"
Evan pepper (Talk | contribs) |
Brittneywick (Talk | contribs) |
||
| (8 intermediate revisions by 2 users not shown) | |||
| Line 36: | Line 36: | ||
h4 { | h4 { | ||
| − | + | font-family: 'objektiv-mk1' !important; | |
| + | font-size: 150%; | ||
| + | font-weight: 400; | ||
} | } | ||
| Line 83: | Line 85: | ||
font-size: 20px; | font-size: 20px; | ||
font-family: "Objektiv-mk1" !important; | font-family: "Objektiv-mk1" !important; | ||
| − | font-weight: | + | font-weight: 400 !important; |
padding: 16px 32px; | padding: 16px 32px; | ||
} | } | ||
| Line 104: | Line 106: | ||
font-size: 20px; | font-size: 20px; | ||
font-family: "Objektiv-mk1" !important; | font-family: "Objektiv-mk1" !important; | ||
| − | font-weight: | + | font-weight: 400 !important; |
padding: 16px 32px; | padding: 16px 32px; | ||
} | } | ||
| Line 307: | Line 309: | ||
h4 { | h4 { | ||
| − | + | font-family: 'objektiv-mk1' !important; | |
| + | font-size: 150%; | ||
| + | font-weight: 400; | ||
} | } | ||
| Line 502: | Line 506: | ||
<br> | <br> | ||
| − | <img class="titleimg" src="https://static.igem.org/mediawiki/2017/3/37/Gloves.png"> | + | <img class="titleimg" src="https://static.igem.org/mediawiki/2017/3/37/Gloves.png" alt="http://freevector.co/vector-icons/fashion/protection-gloves.html"> |
<h1>SAFETY</h1> | <h1>SAFETY</h1> | ||
| Line 510: | Line 514: | ||
<div class="text-container"> | <div class="text-container"> | ||
| − | <p><span class="firstword">When</span> choosing an organism to help us produce acetaminophen and vitamin B<sub>12</sub> we sought out an FDA-approved microbe; however, we resorted to using a non-toxic cyanobacteria as a model for | + | <p><span class="firstword">When</span> choosing an organism to help us produce acetaminophen and vitamin B<sub>12</sub> we sought out an FDA-approved microbe; however, we resorted to using a non-toxic cyanobacteria, <i>Synechococcus elongatus</i> strain PCC 7942, as a model for <i>Arthrospira platensis</i>. <i>A. platensis</i> is a generally recognized as safe organism and our genes were chosen from bio-safety level 1 (BSL-1) organisms.</p> |
| Line 536: | Line 540: | ||
<br> | <br> | ||
| − | <p>We are growing two different strains of <i>S. elongatus</i>: 7002 and 7942. While PCC 7942 will act as our host organism due to its metabolic similarity to <i>A. platensis</i>, we plan to use PCC 7002, which requires external B<sub>12</sub> for survival, as a growth reference for the detection of vitamin B<sub>12</sub>. PCC 7942 was obtained from the University of California, San Diego (UCSD) iGEM team and PCC 7002 was obtained from Dr. Donald Bryant at Pennsylvania State University.</p> | + | <!--<p>We are growing two different strains of <i>S. elongatus</i>: 7002 and 7942. While PCC 7942 will act as our host organism due to its metabolic similarity to <i>A. platensis</i>, we plan to use PCC 7002, which requires external B<sub>12</sub> for survival, as a growth reference for the detection of vitamin B<sub>12</sub>. PCC 7942 was obtained from the University of California, San Diego (UCSD) iGEM team and PCC 7002 was obtained from Dr. Donald Bryant at Pennsylvania State University.</p>--> |
| − | + | ||
| − | + | ||
| − | + | ||
<h3>VITAMIN B<sub>12</sub></h3> | <h3>VITAMIN B<sub>12</sub></h3> | ||
Latest revision as of 23:58, 1 November 2017
SAFETY
When choosing an organism to help us produce acetaminophen and vitamin B12 we sought out an FDA-approved microbe; however, we resorted to using a non-toxic cyanobacteria, Synechococcus elongatus strain PCC 7942, as a model for Arthrospira platensis. A. platensis is a generally recognized as safe organism and our genes were chosen from bio-safety level 1 (BSL-1) organisms.
L A B SAFETY
Proper PPE for BSL-1 organisms are worn in our laboratory. Within the lab space, every person is expected to be wearing gloves, long pants, close-toed shoes, a lab coat, and protective goggles. All personnel also must be accompanied with one other person so that there is no one alone in lab.
P R O J E C T SAFETY
VITAMIN B12
G E N E S U S E D:
I. ssuE --> produces FMN reductase
Organism: Synechoccocous elongatus strain PCC 7002
Biosafety Level I
The synthesis of 5,6-DMB first requires the presence of reduced flavin mononucleotide (FMNH2). FMNH2 is reduced from flavin mononucleotide (FMN) by FMN reductase, which is encoded with the ssuE gene[19,20].
FMNH2 + NADP+ = FMN + NADPH
II. bluB --> 5,6-DMB synthase
Organism: Sinorhizobium meliloti 1021
Biosafety Level I
ACETAMINOPHEN
G E N E S U S E D:
I. 4ABH --> 4-aminophenol
Organism: Agaricus bisporus
Biosafety Level I
II. nhoA --> converts 4-aminophenol into acetaminophen
Organism: Escherichia coli
Biosafety Level I
