Difference between revisions of "Team:UNOTT/Experiments"

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       <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">STEP 1: Create guideRNA Plasmid </h4>
 
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<h1>Why</h1>
 
<p>We first produced our gRNA plasmids in order to stop expression of targeted fluorescent proteins on our reporter plasmid. </p>
 
<p>We first produced our gRNA plasmids in order to stop expression of targeted fluorescent proteins on our reporter plasmid. </p>
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<h1>How</h1>
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<h1>Results</h1>
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            <h3><span>RESULTS</span></h3>
 
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       <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">STEP 2: Create Reporter Plasmid</h4><center><img class="icons" src="https://static.igem.org/mediawiki/2017/e/e7/T--UNOTT--REPORTER.png" style="width:400px;height:auto;"></center>
 
       <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">STEP 2: Create Reporter Plasmid</h4><center><img class="icons" src="https://static.igem.org/mediawiki/2017/e/e7/T--UNOTT--REPORTER.png" style="width:400px;height:auto;"></center>
 
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       <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">STEP 4: Random Ligations</h4><center><img class="icons3" src="https://static.igem.org/mediawiki/2017/d/d6/T--UNOTT--Randomligations.png" style="width:300px;height:auto;"></center>
 
       <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">STEP 4: Random Ligations</h4><center><img class="icons3" src="https://static.igem.org/mediawiki/2017/d/d6/T--UNOTT--Randomligations.png" style="width:300px;height:auto;"></center>
 
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Revision as of 01:46, 1 November 2017





EXPERIMENTS:

STEP 1: Create guideRNA Plasmid

STEP 2: Create Reporter Plasmid

STEP 3: Promoter Library

STEP 4: Random Ligations

STEP 5: Freeze Drying & Revival

STEP 6: CRISPRi & gRNA Efficiency