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<p> The next step in developing our simulation was to calculate our protein concentration at any given time when using CRISPRi. Discussion with wet-lab revealed our method would be using CRISPRi as a repressor, which works by inhibiting the expression of one or more genes by binding to the promoter region <sup> 1 </sup>. The expanded mRNA and Protein concentration models from the Constitutive Gene Expression Model <sup> 2 </sup> were modified to include the element of repression from the CRISPRi inhibition. </p> | <p> The next step in developing our simulation was to calculate our protein concentration at any given time when using CRISPRi. Discussion with wet-lab revealed our method would be using CRISPRi as a repressor, which works by inhibiting the expression of one or more genes by binding to the promoter region <sup> 1 </sup>. The expanded mRNA and Protein concentration models from the Constitutive Gene Expression Model <sup> 2 </sup> were modified to include the element of repression from the CRISPRi inhibition. </p> | ||
− | $$ Gene \overset{Repressor}{\rightarrow} mRNA \rightarrow Protein $$ | + | $$ \color{white} Gene \overset{Repressor}{\rightarrow} mRNA \rightarrow Protein $$ |
$$ mRNA \underset{Degradation}{\rightarrow} \oslash $$ | $$ mRNA \underset{Degradation}{\rightarrow} \oslash $$ |
Revision as of 20:27, 31 October 2017
MODELLING