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<h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">Gene Transcription Regulation by Repressors (CRISPRi) - Concentration over Time</h4><center></center> | <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">Gene Transcription Regulation by Repressors (CRISPRi) - Concentration over Time</h4><center></center> | ||
+ | <h5 style="color: #C0C0C0; font-weight: bold; font-size: 20px;"> Calculating how much protein is produced over time when a gene is inhibited </h5> | ||
+ | |||
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<p> The next step in developing our simulation was to calculate our protein concentration at any given time when using CRISPRi. Discussion with wet-lab revealed our method would be using CRISPRi as a repressor, which works by inhibiting the expression of one or more genes by binding to the promoter region <sup> 1 </sup>. The expanded mRNA and Protein concentration models from the Constitutive Gene Expression Model <sup> 2 </sup> were modified to include the element of repression from the CRISPRi inhibition. </p> | <p> The next step in developing our simulation was to calculate our protein concentration at any given time when using CRISPRi. Discussion with wet-lab revealed our method would be using CRISPRi as a repressor, which works by inhibiting the expression of one or more genes by binding to the promoter region <sup> 1 </sup>. The expanded mRNA and Protein concentration models from the Constitutive Gene Expression Model <sup> 2 </sup> were modified to include the element of repression from the CRISPRi inhibition. </p> | ||
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<h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">Relationship between Max Fluorescence and Protein Concentration</h4> | <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">Relationship between Max Fluorescence and Protein Concentration</h4> | ||
+ | <h5 style="color: #C0C0C0; font-weight: bold; font-size: 20px;"> Using our models to estimate the amount of fluorescence expected from a certain concentration of protein synthesized </h5> | ||
<center></center> | <center></center> | ||
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<h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">Are Our Constructions Random?</h4><center></center> | <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">Are Our Constructions Random?</h4><center></center> | ||
+ | <h5 style="color: #C0C0C0; font-weight: bold; font-size: 20px;"> Showing that our constructions are random and why they are random </h5> | ||
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<p> When constructing our proteins with our current method, there were 3 vectors we could order from. <p> | <p> When constructing our proteins with our current method, there were 3 vectors we could order from. <p> |
Revision as of 23:34, 31 October 2017
MODELING