MattAFrench (Talk | contribs) |
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<p> This system can be described as above. Where gRNA(i), Cas9, and mRNA are produced constitutively with their associated rates of production kc, kg, and k0 respectively. The Cas9 and gRNA(i) will undergo an inrreversible association to form Cas9:gRNA(i) at rate kf, which in turn inhibits the production of mRNA and reduce the production of Fluorescent protein (k1). All molecules spontaneously degrade and diffuse away at their own ssociated rate. (i) will account for us having multiple gRNAs and just as many fluorescent proteins i.e. i=3 with three fluorescent proteins and subsequent set of three gRNAs.</p> | <p> This system can be described as above. Where gRNA(i), Cas9, and mRNA are produced constitutively with their associated rates of production kc, kg, and k0 respectively. The Cas9 and gRNA(i) will undergo an inrreversible association to form Cas9:gRNA(i) at rate kf, which in turn inhibits the production of mRNA and reduce the production of Fluorescent protein (k1). All molecules spontaneously degrade and diffuse away at their own ssociated rate. (i) will account for us having multiple gRNAs and just as many fluorescent proteins i.e. i=3 with three fluorescent proteins and subsequent set of three gRNAs.</p> | ||
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− | <p>The system can be described by the following 5 ordinary differential equations, defining how the concentration of each variable will change at any given change in time. Equations 1, 2 and 3 are derived from Farasat <i>et al.</i>(2016), which comprehensively investigated the rates at which CRISPR-Cas9 can cleave DNA targets. | + | <p>The system can be described by the following 5 ordinary differential equations, defining how the concentration of each variable will change at any given change in time. Equations 1, 2 and 3 are derived from Farasat <i>et al.</i>(2016), which comprehensively investigated the rates at which CRISPR-Cas9 can cleave DNA targets.</p> |
− | + | <h1> $$ \color{white}{(1) \frac{dgRNA,i}{dt} = k_{g,i} – δ_{dg} \cdot gRNA,i – k_{f} \cdot Cas9 \cdot gRNA,i} $$</h1> | |
<p style="text-align: center;" > | <p style="text-align: center;" > | ||
The above equation details the change in gRNA concentration per unit time, also extending along index i. At any given time, the concentration of gRNA(i) will be increased by its production (kgi), and decreased by its association with cas9 at rate kf, relative to it's concentration, and it will also degrade and diffuse away at rate δdg.</p><br><br> | The above equation details the change in gRNA concentration per unit time, also extending along index i. At any given time, the concentration of gRNA(i) will be increased by its production (kgi), and decreased by its association with cas9 at rate kf, relative to it's concentration, and it will also degrade and diffuse away at rate δdg.</p><br><br> |
Revision as of 01:33, 1 November 2017
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