MattAFrench (Talk | contribs) |
MattAFrench (Talk | contribs) |
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− | + | $$ \color{white}{(1) \frac{dgRNA,i}{dt} = k_{g,i} – δ_{dg} \cdot gRNA,i – k_{f} \cdot Cas9 \cdot gRNA,i} $$ | |
<p style="text-align: center;" > | <p style="text-align: center;" > | ||
The above equation details the change in gRNA concentration per unit time, also extending along index i. At any given time, the concentration of gRNA(i) will be increased by its production (kgi), and decreased by its association with cas9 at rate kf, relative to it's concentration, and it will also degrade and diffuse away at rate δdg.</p><br><br> | The above equation details the change in gRNA concentration per unit time, also extending along index i. At any given time, the concentration of gRNA(i) will be increased by its production (kgi), and decreased by its association with cas9 at rate kf, relative to it's concentration, and it will also degrade and diffuse away at rate δdg.</p><br><br> | ||
− | + | $$ \color{white}{(2) \frac{dCas9}{dt} = k_{c} – δ_{dc} \cdot Cas9 – k_{f} \cdot Cas9 \cdot \underset{i}{∑}gRNA,i} $$ | |
<p style="text-align: center;" > This equation details the change in Cas9 protein per unit time. It will be increased by its production (kc) and reduced by its degradation (δdc), and again it's association to gRNA(s). This will be proportioal the sum of all the gRNA's along i, accounting for the competition for Cas9.</p><br><br> | <p style="text-align: center;" > This equation details the change in Cas9 protein per unit time. It will be increased by its production (kc) and reduced by its degradation (δdc), and again it's association to gRNA(s). This will be proportioal the sum of all the gRNA's along i, accounting for the competition for Cas9.</p><br><br> | ||
− | + | $$ \color{white}{(3) \frac{dCas9:gRNA,i}{dt} = k_{f} \cdot Cas9:gRNA,i – δ_{dcg} } $$ | |
<p style="text-align: center;" > This equation details the change in concentration of the Cas9 associated with gRNA(i). This is simply the rate of formation from before, minus its degredation. </p><br><br> | <p style="text-align: center;" > This equation details the change in concentration of the Cas9 associated with gRNA(i). This is simply the rate of formation from before, minus its degredation. </p><br><br> | ||
− | + | $$ \color{white}{(4) \frac{dmRNA,i}{dt} = k_{0} \cdot \frac{1}{1+k{m} \cdot Cas9:gRNA,i} −δ_{dm} \cdot mRNA,i} $$ | |
<p style="text-align: center;" > This equation details the change in mRNA(i), which is very similar to the equation seen earlier when describing transciption. This is produced at a rate k0, but it is also inhibited by Cas9:grna(i), so there is a standard inhibition function which will reduce rate k0 as Cas9:gRNA(i) increases. It is also simply reduced by it's degradation and diffusion rate δdm. </p><br><br> | <p style="text-align: center;" > This equation details the change in mRNA(i), which is very similar to the equation seen earlier when describing transciption. This is produced at a rate k0, but it is also inhibited by Cas9:grna(i), so there is a standard inhibition function which will reduce rate k0 as Cas9:gRNA(i) increases. It is also simply reduced by it's degradation and diffusion rate δdm. </p><br><br> | ||
− | <font size="10"> $$ \color{white}{(5) \frac{dFP,i}{dt} = k_{1} \cdot mRNA,i – δ_{dp} \cdot FP,i} $$ | + | <font size="10"> $$ \color{white}{(5) \frac{dFP,i}{dt} = k_{1} \cdot mRNA,i – δ_{dp} \cdot FP,i} $$ |
<p style="text-align: center;" > This details the rate of translation and is the same as before; only changes to protein translation are increased proportionally to mRNA(i) and reduced by it's degradation and diffusion δdp. <sup> 3 </sup> : </p><br><br> | <p style="text-align: center;" > This details the rate of translation and is the same as before; only changes to protein translation are increased proportionally to mRNA(i) and reduced by it's degradation and diffusion δdp. <sup> 3 </sup> : </p><br><br> | ||
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<p> The value for these constants and variables were taken from literature and calculating them <sup> 4 </sup> but later, adjusted to the lab results.</p> | <p> The value for these constants and variables were taken from literature and calculating them <sup> 4 </sup> but later, adjusted to the lab results.</p> |
Revision as of 01:43, 1 November 2017
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