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Revision as of 19:03, 1 November 2017





MODELLING

Overview







About modeling and why iGEM Nottingham chose to do it

Constitutive Gene Expression For Protein and mRNA Expression over Time

The general gene expression equation showing the process of protein synthesis

Gene Transcription Regulation by Repressors (CRISPRi) - Concentration over Time

Calculating how much protein is produced over time when a gene is inhibited

Relationship between Max Fluorescence and Protein Concentration

Using our models to estimate the amount of fluorescence expected from a certain concentration of protein synthesized

Absorption and Emission Wavelengths From Given Concentrations of sfGFP, mRFP & ECFP

Working out which wavelengths are required to produce a fluorescence spectra.

Are Our Constructions Random?



Showing that our constructions are random and why they are random

Conclusion

What iGEM Nottingham 2017 learnt from modelling and how modelling impacted the project.