Difference between revisions of "Team:UT-Knoxville"
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<li><a href="https://2017.igem.org/Team:UT-Knoxville">HOME</a></li><li> | <li><a href="https://2017.igem.org/Team:UT-Knoxville">HOME</a></li><li> | ||
<a href="https://2017.igem.org/Team:UT-Knoxville/Team">TEAM</a></li><li> | <a href="https://2017.igem.org/Team:UT-Knoxville/Team">TEAM</a></li><li> | ||
| − | <a href="https:// | + | <a href="https://2017.igem.org/Team:UT-Knoxville/Project">PROJECT</a> |
| − | <ul> | + | <ul> |
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Project#Motivation">Motivation</a></li> |
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Project#PuPromoter">Pu Promoter</a></li> |
| − | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Project#ToxicityEffluxPumps">Toxicity Efflux Pumps</a></li> | |
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Project#EnzymeHomologues">Enzyme Homologues</a></li> |
| − | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Project#References">References</a></li> | |
| − | + | </ul> | |
| − | + | ||
| − | + | ||
| − | </ul> | + | |
</li><li> | </li><li> | ||
<a href="#">NOTEBOOK</a> | <a href="#">NOTEBOOK</a> | ||
<ul> | <ul> | ||
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Calendar">Calendar</a></li> |
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Methods">Methods</a></li> |
<li><a href="https://2017.igem.org/Team:UT-Knoxville/Protocols">Protocols</a></li> | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Protocols">Protocols</a></li> | ||
</ul> | </ul> | ||
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<a href="#">ATTRIBUTIONS</a> | <a href="#">ATTRIBUTIONS</a> | ||
<ul> | <ul> | ||
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Sponsors">Sponsors</a></li> |
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Attributions">Group</a></li> |
</ul> | </ul> | ||
</li><li> | </li><li> | ||
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<ul> | <ul> | ||
<li><a href="https://2017.igem.org/Team:UT-Knoxville/Engagement">Engagement</a></li> | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Engagement">Engagement</a></li> | ||
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/HP/Silver">Silver HP Requirement</a></li> |
</ul> | </ul> | ||
</li><li> | </li><li> | ||
<a href="#"> JUDGING</a> | <a href="#"> JUDGING</a> | ||
<ul> | <ul> | ||
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Proof">Medal Requirements</a></li> |
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Collaborations">Collaborations</a></li> |
| − | <li><a href="https:// | + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Interlab">Interlab</a></li> |
| + | <li><a href="https://2017.igem.org/Team:UT-Knoxville/Parts">Submitted Parts</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
</ul> | </ul> | ||
</nav> | </nav> | ||
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<div class="main"> | <div class="main"> | ||
| − | <h3 | + | <center><h3 style="padding:10px; margin:50px 70px 0px 80px;"><b> ABSTRACT </b></h3></center> |
| − | <p class="para"> Crude oil processing produces toxic byproducts, such as benzene, toluene, and xylenes (BTX) capable of contaminating groundwater and soil leading to potentially serious health risks for both humans and wildlife. Thus, the effective removal of these hazardous organic compounds is imperative towards protecting natural resources. While it is possible to manually clean contaminated sites, such efforts are costly and time consuming. Our project seeks to degrade the toluene-based contaminants into valuable aromatic aldehydes by expressing the xyl ortho pathway from Pseudomonas putida in E. coli. As a continuation of our 2016 project, the 2017 UT-Knoxville iGEM team aims to use synthetic biology and metabolic engineering techniques to further develop our bioremediation strain. First, regulatory elements sensitive to the presence of aromatic hydrocarbons are engineered to fine-tune protein production. Second, overexpression of broadly specific efflux pumps intend to increase organic solvent tolerance and alleviate toxic effects. And third, exploring enzymatic homologs can expand our product library. Our project aims to increase production titers and develop a more robust microorganism suitable for manufacturing and bioremediation needs. | + | <p class="para"> Crude oil processing produces toxic byproducts, such as benzene, toluene, and xylenes (BTX) capable of contaminating groundwater and soil leading to potentially serious health risks for both humans and wildlife. Thus, the effective removal of these hazardous organic compounds is imperative towards protecting natural resources. While it is possible to manually clean contaminated sites, such efforts are costly and time consuming. Our project seeks to degrade the toluene-based contaminants into valuable aromatic aldehydes by expressing the xyl ortho pathway from <i>Pseudomonas putida</i> in <i>E. coli</i>. As a continuation of our 2016 project, the 2017 UT-Knoxville iGEM team aims to use synthetic biology and metabolic engineering techniques to further develop our bioremediation strain. First, regulatory elements sensitive to the presence of aromatic hydrocarbons are engineered to fine-tune protein production. Second, overexpression of broadly specific efflux pumps intend to increase organic solvent tolerance and alleviate toxic effects. And third, exploring enzymatic homologs can expand our product library. Our project aims to increase production titers and develop a more robust microorganism suitable for manufacturing and bioremediation needs. |
</p> | </p> | ||
| − | <center><h4 | + | <center><h4 style="padding:10px;margin:50px 70px 0px 80px;"><b> UT-Knoxville 2016 Project Overview </b></h4></center> |
<table> | <table> | ||
<tr> | <tr> | ||
<td> | <td> | ||
<img src="https://static.igem.org/mediawiki/2016/6/6d/Refinery.png" class="refinery"> | <img src="https://static.igem.org/mediawiki/2016/6/6d/Refinery.png" class="refinery"> | ||
| − | + | </td> | |
| − | <td> | + | <td style="display:block;position:relative;top:-200px;" rowspan="2"> |
<ul> | <ul> | ||
| − | <li | + | <li> Crude oil processing produces toxic byproducts, such as benzene, toluene, and xylenes.</li> |
<br> | <br> | ||
| − | <li | + | <li> These byproducts can contaminate the groundwater and soil.</li> |
</ul> | </ul> | ||
</td> | </td> | ||
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<td> | <td> | ||
<img src="https://static.igem.org/mediawiki/2016/0/04/Ecoli2.png" class="ecoli"> | <img src="https://static.igem.org/mediawiki/2016/0/04/Ecoli2.png" class="ecoli"> | ||
| − | + | </td> | |
| − | <td> | + | <td style="display:block;position:relative;top:-200px;" rowspan="2"> |
<ul> | <ul> | ||
| − | <li | + | <li> These harmful byproducts can be converted to useful products in a renewable, environmentally-friendly, and high-yield manner via <i>E. coli</i>. </li> |
<br> | <br> | ||
| − | <li | + | <li> The TOL pathway in <i>P. putida</i> can convert these byproducts into aromatic aldehydes. </li> |
</ul> | </ul> | ||
</td> | </td> | ||
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<td> | <td> | ||
<img src="https://static.igem.org/mediawiki/2016/9/97/Products.png" class="products"> | <img src="https://static.igem.org/mediawiki/2016/9/97/Products.png" class="products"> | ||
| − | + | </td> | |
| − | <td> | + | <td style="display:block;position:relative;top:-200px;" rowspan="2"> |
<ul> | <ul> | ||
| − | <li | + | <li> The aromatic aldehyde products are used in the fragrance and pharmaceutical industries. </li> |
</ul> | </ul> | ||
</td> | </td> | ||
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Latest revision as of 00:04, 2 November 2017
<!DOCTYPE html>
Team:UT-Knoxville/Team
UTK
iGEM
ABSTRACT
Crude oil processing produces toxic byproducts, such as benzene, toluene, and xylenes (BTX) capable of contaminating groundwater and soil leading to potentially serious health risks for both humans and wildlife. Thus, the effective removal of these hazardous organic compounds is imperative towards protecting natural resources. While it is possible to manually clean contaminated sites, such efforts are costly and time consuming. Our project seeks to degrade the toluene-based contaminants into valuable aromatic aldehydes by expressing the xyl ortho pathway from Pseudomonas putida in E. coli. As a continuation of our 2016 project, the 2017 UT-Knoxville iGEM team aims to use synthetic biology and metabolic engineering techniques to further develop our bioremediation strain. First, regulatory elements sensitive to the presence of aromatic hydrocarbons are engineered to fine-tune protein production. Second, overexpression of broadly specific efflux pumps intend to increase organic solvent tolerance and alleviate toxic effects. And third, exploring enzymatic homologs can expand our product library. Our project aims to increase production titers and develop a more robust microorganism suitable for manufacturing and bioremediation needs.
UT-Knoxville 2016 Project Overview
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