Team:UT-Knoxville/Parts
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Team:UT-Knoxville/Team
UTK
iGEM
Parts Collection
UT-Knoxville 2017 has submitted the following parts:
The xylR coding sequence was synthesized during our 2016 project, and the Pu promoter was amplified from 2013 Peking’s part BBa_K1031803. All constructs were made by Gibson Assembly. Confirmed sequences of these parts are available on the registry.
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Each gene of interest was amplified from E. coli MG1655 genomic DNA, and Gibson Assembled into a linearized vector consisting of pSB1C3, BBa_B0034, and BBa_B0010. The constructs were first transformed into Top10, and characterized and sequenced in BL21DE3. Confirmed sequences of these parts are available in the registry.
Note: pRob (BBa_K2451006) remains BioBrick incompatible due to EcoRI and PstI in the coding sequence.
