Difference between revisions of "Team:Uppsala/Demonstrate"

In our project we have focused on extending the zeaxanthine pathway to produce compounds along the way to crocin. We have stabilized the expression of zeaxanthin by lambda red recombineering and produced zeaxanthin. The next goal was to introduce further steps in the pathway. In the first step we included the enzyme CaCDD2 with a lac promotor. The enzyme construct was verified by successful sequencing. Then we introduced another step: CsADH2946, the enzyme that leads to Crocetin. Crocetin is a biologically interesting and research compound. We have successfully expressed and purified this enzyme and tested its activity. Both CaCDD2 and CsADH2946 were transformed into the zeaxanthin strain and production of crocetin was observed by color change. Our third step was to produce crocin. This is done with enzyme called UGTCs2. Again after creating the biobrick and transforming we have sequenced the plasmid succesfully. We have also transformed the combined BioBrick with CaCCD2, CsADH2946 and UGTCs2 into the zeaxanthin strain.

You can see all of our results on our Results page