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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Wednesday, October 11</u></strong></p> | <p><strong><u>Wednesday, October 11</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Collin</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Made Kanamycin plates <em>[Collin]</em></li> | |
+ | <li>Prepared bacterial stabs of Dsup, phrAC, and phrAT for the UChicago team <em>[Collin]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<p><strong><u>Thursday, October 12</u></strong></p> | <p><strong><u>Thursday, October 12</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<p><strong><u>Friday, October 13</u></strong></p> | <p><strong><u>Friday, October 13</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<p><strong><u>Saturday, October 14</u></strong></p> | <p><strong><u>Saturday, October 14</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<p><strong><u>Sunday, October 15</u></strong></p> | <p><strong><u>Sunday, October 15</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Monday, October 16</u></strong></p> | <p><strong><u>Monday, October 16</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Mark</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Gel purified pSB1C3 and nptII <em>[Mark]</em></li> | |
+ | <li>Ligated pSB1C3 and nptII <em>[Mark]</em></li> | ||
+ | <li>Transformed pSB1C3+nptII <em>[Mark]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<p><strong><u>Tuesday, October 17</u></strong></p> | <p><strong><u>Tuesday, October 17</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<p><strong><u>Wednesday, October 18</u></strong></p> | <p><strong><u>Wednesday, October 18</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Thursday, October 19</u></strong></p> | <p><strong><u>Thursday, October 19</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Collin</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Streaked phrAT, phrAC, BCP, uvsE, and BCP <em>[Collin]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Friday, October 20</u></strong></p> | <p><strong><u>Friday, October 20</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Collin</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Transformed lac+RBS+Dsup+BCP into E.coli cells <em>[Collin]</em></li> | |
+ | <li>Prepared cultures of lac+RBS+Dsup, lac+RBS+BCP, and lac+RBS+uvsE <em>[Collin]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 2,894: | Line 2,874: | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Saturday, October 21</u></strong></p> | <p><strong><u>Saturday, October 21</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Micah</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Transformed medium promoter+medium RBS (BBa_K608006) into E. coli <em>[Micah]</em></li> | |
+ | <li>Prepared cultures from previous transformation <em>[Micah]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 2,907: | Line 2,888: | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Sunday, October 22</u></strong></p> | <p><strong><u>Sunday, October 22</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present:Collin</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Made glycerol stocks of lac+RBS+Dsup+RBS+BCP+pSB1C3 <em>[Collin]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Monday, October 23</u></strong></p> | <p><strong><u>Monday, October 23</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Collin</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Miniprepped lac+RBS+BCP+pSB1C3 <em>[Collin]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 2,933: | Line 2,914: | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Tuesday, October 24</u></strong></p> | <p><strong><u>Tuesday, October 24</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Collin, Mark</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Prepared two overnights of Dsup and one of BCP <em>[Collin]</em></li> | |
+ | <li>Digested nptII and med promoter + med RBS + pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Ran gel electrophoresis on the digested DNA <em>[Mark]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Wednesday, October 25</u></strong></p> | <p><strong><u>Wednesday, October 25</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present:Mark, Collin, Micah, Maddie, Zoe</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Performed the UV irradiation test on Dsup and the control BCP <em>[Micah, Collin, Maddie, Zoe]</em></li> | |
+ | <li>Gel purified nptII and med promoter + med RBS + pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Ligated nptII to the med promoter + med RBS + pSB1C3 <em>[Mark, Collin]</em></li> | ||
+ | <li>Transformed the ligated DNA into E. coli cells <em>[Collin, Mark]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 2,959: | Line 2,945: | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Thursday, October 26</u></strong></p> | <p><strong><u>Thursday, October 26</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Micah</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Prepared an overnight culture of med promoter + med RBS + nptII <em>[Micah]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 2,972: | Line 2,958: | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Friday, October 27</u></strong></p> | <p><strong><u>Friday, October 27</u></strong></p> | ||
− | <p><em></em></p> | + | <p><em>Present: Micah, Alex</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Miniprepped the med promoter + med RBS + nptII <em>[Micah]</em></li> | |
+ | <li>Prepared the DNA to be sent to the registry <em>[Alex, Micah]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<p><strong><u>Saturday, October 28</u></strong></p> | <p><strong><u>Saturday, October 28</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<p><strong><u>Sunday, October 29</u></strong></p> | <p><strong><u>Sunday, October 29</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | + | <p>No work done today!</p> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
Line 3,012: | Line 2,991: | ||
<p><strong><u>Monday, October 30</u></strong></p> | <p><strong><u>Monday, October 30</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
Line 3,025: | Line 3,000: | ||
<p><strong><u>Tuesday, October 31</u></strong></p> | <p><strong><u>Tuesday, October 31</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
− | <p> | + | <p>No work done today!</p> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> |
Latest revision as of 03:17, 2 November 2017
Notebook
Click on a date to see what we did on that day!
June 2017 | ||||||
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Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday |
1 | 2 | 3 | ||||
4 | 5 | 6 | 7 | 8 | 9 | 10 |
11 | 12 | 13 | 14 | 15 | 16 | 17 |
18 | 19 | 20 | 21 | 22 | 23 | 24 |
25 | 26 | 27 | 28 | 29 | 30 | |